Diversity and detection of Korean Erwinia pyrifoliae strains as determined by plasmid profiling, phylogenetic analysis and PCR |
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| Authors: | R Shrestha † S H Lee J E Kim C Wilson S-G Choi D H Park M H Wang J H Hur C K Lim |
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| Institution: | Division of Bio-Resources Technology, College of Agriculture and Life Sciences, Kangwon National University (KNU), Chuncheon 200-701, Republic of Korea;;Department of Agricultural Sciences, School of Agricultural Sciences, University of Tasmania, Hobart, TAS 7001, Australia;;Department of Bioengineering and Technology, College of Engineering, KNU, Chuncheon 200-701;;Division of Biotechnology, KNU, Chuncheon 200-701;and;Division of Biological Environment, College of Agriculture and Life Sciences, KNU, Chuncheon 200-701, Republic of Korea |
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| Abstract: | Twenty-five strains of Erwinia pyrifoliae were investigated for their plasmid profiles and genetic relatedness. Four types of plasmid profile were observed for the first time, suggesting intraspecific plasmid profile diversity in E. pyrifoliae . Moreover, BOX-PCR and phylogenetic analysis based on the 16S-23S intergenic transcribed spacer (ITS) region showed genetic variations among E. pyrifoliae strains, although all strains were clustered in one group and separated from E. amylovora . On the other hand, ERIC-PCR and phylogenetic analysis based on partial groEL gene sequences revealed close genetic relatedness among the strains. Amplification with EpSPF and EpSPR primers of a fragment of approximately 0·65 kb from the genomic DNA of all E. pyrifoliae strains, but not from E. amylovora strains, suggested that this primer set is useful for identification of this pathogen. |
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| Keywords: | ERIC-PCR groEL gene plasmid profile Pyrus pyrifolia shoot blight |
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