| CD58及雌酮对妊娠早期山羊EML分泌IL—2及IL—4的影响 |
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| 引用本文： | 沈文正,王爱华,李引乾. CD58及雌酮对妊娠早期山羊EML分泌IL—2及IL—4的影响[J]. 西北农林科技大学学报(自然科学版), 2002, 30(6): 52-54 |
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| 作者姓名： | 沈文正 王爱华 李引乾 |
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| 作者单位： | 1. 杨凌职业技术学院,动物工程系,陕西,杨陵,712100
2. 西北农林科技大学,国家重点开放家畜生殖内分泌实验室,陕西,杨陵,712100 |
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| 基金项目： | 国家自然科学基金资助项目(39770545) |
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| 摘 要： | 将妊娠17d山羊子宫内膜淋巴细胞(EML)放入含不同剂量PHA-P,CD58和雌酮的介质中进行体外培养,并分别用生物学方法和双抗体夹心ELISA法测定培养上清液中IL-2和IL-4的水平。结果表明,CD58和雌酮处理组EML分泌的IL-2水平低,与细胞对照组差异不显著,并显著低于PHA-P处理组。3个浓度CD58处理组IL-4分泌水平高达5.40～6.36ng/mL,高浓度雌酮处理组IL-4分泌量也达到4.77ng/mL,与细胞对照组差异均极显著,而且CD58能以剂量依赖方式促进EML对IL-4的分泌。
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| 关 键 词： | 子宫内膜淋巴细胞(EML) IL-2 IL-4 CD58 雌酮 生殖调控 |
| 文章编号： | 1671-9387(2002)06-0052-03 |
| 收稿时间： | 2001-11-14 |
| 修稿时间： | 2001-11-14 |
Effects of CD58 and estrone on the secretion of IL-2 and IL-4 by goat EML in early gestation |
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| Affiliation: | SHEN Wen zheng1,WANG Ai hua2,LI Yin qian2,MA Yong jiang2,GUO Xin yi1,MA An liang1,ZHANG Zhen cang1(1 Yangling Vocational and Technical College,Yangling,Shaanxi 712100,China;2 Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China) |
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| Abstract: | Endometrial lymphocytes (EMLs) of pregnant goat (17d) were cultured in vitro respectively in media containing PHA-P,CD58 and estrone at different doses.Then IL-2 and IL-4 in medium supernatants were measured respectively with biological method and double-antibody ELISA.The results showed that EMLs treated with CD58 and estrone secreted IL-2 at low level are insignificantly different from that of control cell group and significantly lower than that of EMLs treated with PHA-P.The EMLs treated with CD58 in three concentrations dose-dependently produced 5.40-6.36 ng/mL of IL-4 and those with estrone at higher dose produced 4.77 ng/mL of IL-4,all significantly higher than that of control cell group. |
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| Keywords: | EML IL-2 IL-4 CD58 estrone reproductive control |
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