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| Isolating Soil Drought-Induced Genes from Maize Seedling Leaves Through Suppression Subtractive Hybridization | |
| 引用本文： | LI Hui-yong HUANG Su-hua SHI Yun-su SONG Yan-chun ZHAO Jiu-ran WANG Feng-ge WANG Tian-yu LI Yu. Isolating Soil Drought-Induced Genes from Maize Seedling Leaves Through Suppression Subtractive Hybridization[J]. 中国农业科学(英文版), 2007, 6(6): 647-651. DOI: 10.1016/S1671-2927(07)60095-9 |
| 作者姓名： | LI Hui-yong HUANG Su-hua SHI Yun-su SONG Yan-chun ZHAO Jiu-ran WANG Feng-ge WANG Tian-yu LI Yu |
| 作者单位： | [1]Institute of Food Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China [2]Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China [3]Maize Research Institute, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100081, P.R.China |
| 基金项目： | 国家自然科学基金;北京自然科学基金农业科技创新平台资助项目 |
| 摘 要： | In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH） library, 672 positive clones were picked up randomly. After polymerase chain reaction （PCR） of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress. |
| 关 键 词： | 玉米苗 叶片 干旱胁迫 基因分离 抑制消减杂交法 |
Isolating Soil Drought-Induced Genes from Maize Seediing Leaves Through Suppression Subtractive Hybridization |
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| LI Hui-yong,HUANG Su-hua,SHI Yun-su,SONG Yan-chun,ZHAO Jiu-ran,WANG Feng-ge,WANG Tian-yu,LI Yu. Isolating Soil Drought-Induced Genes from Maize Seediing Leaves Through Suppression Subtractive Hybridization[J]. 《Agricultural Sciences in China》, 2007, 6(6): 647-651. DOI: 10.1016/S1671-2927(07)60095-9 | |
| Authors: | LI Hui-yong HUANG Su-hua SHI Yun-su SONG Yan-chun ZHAO Jiu-ran WANG Feng-ge WANG Tian-yu LI Yu |
| Affiliation: | 1. Institute of Food Crops, Henan Academy of Agricultural Sciences, Zhengihou 450002, P.R.China;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 3. Maize Research Institute, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100081, P.R.China |
| Abstract: | In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization (SSH) library, 672 positive clones were picked up randomly. After polymerase chain reaction (PCR) of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress. |
| Keywords: | maize drought stress suppression subtractive hybridization (SSH) uniESTs |
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