| Correlation Analysis on Single Nucleotide Polymorphism of CAPN1 Gene and Meat Quality and Carcass Traits in Chickens |
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| 引用本文： | ZHANG Zeng-rong ZHU Qing LIU Yi-ping. Correlation Analysis on Single Nucleotide Polymorphism of CAPN1 Gene and Meat Quality and Carcass Traits in Chickens[J]. 中国农业科学(英文版), 2007, 6(6): 749-754. DOI: 10.1016/S1671-2927(07)60108-4 |
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| 作者姓名： | ZHANG Zeng-rong ZHU Qing LIU Yi-ping |
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| 作者单位： | College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, P.R.China |
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| 基金项目： | The authors acknowledge Li Qinyun, Li Xiaochen, and Jiang Xiaoyu for their help in managing the birds and collecting the data. This work was financially supported by Bio-Tech Programs of Sichuan Province, China （05NG002-017）. |
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| 摘 要： | The selection of meat quality has received considerable focus in chicken breeding. This study was aimed at investigating the effect of CAPN1 gene on meat quality traits in chicken populations. Primer pairs for 3 UTR in CAPN1 were designed from database of chicken genomic sequence. Polymorphisms were detected using PCR-SSCP and DNA sequencing. A mutation at position 9 950 nt （G/A, locus A） was found among individuals in each population. The allele and genotype frequencies significantly differed among eight lines with higher frequencies of allele A2 and genotype AIA2 （P〈0.01）. The least square analysis showed that there was significant difference （P 〈 0.05） in muscle fiber density and some carcass traits among genotypes and that the breast muscle fiber density （BFD） of birds ofAIA1 genotype was significantly higher （P〈0.05） than that of birds ofA2A2 genotype. It was concluded that the CAPN1 gene was the major gene affecting the muscle fiber traits of chicken or was linked with the major gene. These results were useful for studying the molecular mechanism that influences meat traits and were used as the base of molecular-assisted selection to meat quality traits. So, this site may be a potential marker affecting the muscle traits of chickens.
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| 关 键 词： | 肉鸡 CAPN1基因 单核苷酸多态性 肌肉品质 胴体性状 相关分析 |
| 收稿时间： | 2006-11-22 |
| 修稿时间： | 2006-11-22 |
Correlation Analysis on Single Nucleotide Polymorphism of CAPN1 Gene and Meat Quality and Carcass Traits in Chickens |
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| ZHANG Zeng-rong,ZHU Qing,LIU Yi-ping. Correlation Analysis on Single Nucleotide Polymorphism of CAPN1 Gene and Meat Quality and Carcass Traits in Chickens[J]. 《Agricultural Sciences in China》, 2007, 6(6): 749-754. DOI: 10.1016/S1671-2927(07)60108-4 |
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| Authors: | ZHANG Zeng-rong ZHU Qing LIU Yi-ping |
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| Affiliation: | College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, P.R.China |
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| Abstract: | The selection of meat quality has received considerable focus in chicken breeding. This study was aimed at investigating the effect of CAPN1 gene on meat quality traits in chicken populations. Primer pairs for 3'UTR in CAPN1 were designed from database of chicken genomic sequence. Polymorphisms were detected using PCR-SSCP and DNA sequencing. A mutation at position 9 950 nt (G/A, locus A) was found among individuals in each population. The allele and genotype frequencies significantly differed among eight lines with higher frequencies of allele A2 and genotype A1A2 (P＜0.01). The least square analysis showed that there was significant difference (P＜0.05) in muscle fiber density and some carcass traits among genotypes and that the breast muscle fiber density (BFD) of birds of A1A1 genotype was significantly higher (P＜0.05) than that of birds of A2A2 genotype. It was concluded that the CAPN1 gene was the major gene affecting the muscle fiber traits of chicken or was linked with the major gene. These results were useful for studying the molecular mechanism that influences meat traits and were used as the base of molecular-assisted selection to meat quality traits. So, this site may be a potential marker affecting the muscle traits of chickens. |
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| Keywords: | chicken muscle fiber carcass traits CAPN1 SNPs |
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