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| Transfer and Detection of barstarGene to Maize Inbred Line 18-599 （White） by Particle Bombardment | |
| 作者姓名： | SUN Qing-quan ZHANG Ying RONG Ting-zhao DONG Shu-ting ZUO Zhen-peng |
| 作者单位： | [1]Shandong Agricultural University/State Key Laboratory of Crop Biology, Taian 271018, P.R.China [2]Sichuan Agricultural University, Yaan 625014, P.R.China |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 （White）, which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology. |
| 关 键 词： | 玉米 纯系 barstar基因 基因转染 基因检测 粒子轰击法 |
| 收稿时间： | 2 June 2006 |
| 修稿时间： | 2006-06-022006-11-11 |
Transfer and Detection of barstar Gene to Maize Inbred Line 18–599 (White) by Particle Bombardment |
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| SUN Qing-quan ZHANG Ying RONG Ting-zhao DONG Shu-ting ZUO Zhen-peng.Transfer and Detection of barstar Gene to Maize Inbred Line 18–599 (White) by Particle Bombardment[J].Agricultural Sciences in China,2007,6(6):652-656. | |
| Authors: | SUN Qing-quan ZHANG Ying RONG Ting-zhao DONG Shu-ting ZUO Zhen-peng |
| Institution: | 1. Shandong Agricultural University/State Key Laboratory of Crop Biology, Taian 271018, P.R.China;Sichuan Agricultural University, Yaan 625014, P.R.China 2. Sichuan Agricultural University, Yaan 625014, P.R.China 3. Shandong Agricultural University/State Key Laboratory of Crop Biology, Taian 271018, P.R.China |
| Abstract: | In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 (White), which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology. |
| Keywords: | maize inbred line Barstar gene particle bombardment transgenic plant molecular detection |
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