| Studies on Prunus Necrotic Ringspot Virus （PNRSV） Occurring on Lily |
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| 作者姓名： | HAN Li-juan LIU Wen-hong |
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| 作者单位： | [1]Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100025, P.R.China [2]Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, P.R.China |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | This study was to molecular identify Prunus necrotic ringspot virus （PNRSV） occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV. An expected cDNA fragment of about 450 bp was amplified from lily, cloned into pGEM-T-easy vector, sequenced, and shared 84.5-99.1% homology with the 27 PNRSV isolates reported previously at the nucleotide level, indicating that lily is a new natural host of PNRSV.
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| 关 键 词： | 百合 环斑病毒 核糖核酸 鉴定 |
Studies on Prunus Necrotic Ringspot Virus (PNRSV) Occurring on Lily |
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| HAN Li-juan LIU Wen-hong.Studies on Prunus Necrotic Ringspot Virus (PNRSV) Occurring on Lily[J].Agricultural Sciences in China,2007,6(10):1201-1208. |
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| Authors: | HAN Li-juan LIU Wen-hong |
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| Institution: | 1. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100025, P.R. China
2. Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, P.R. China |
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| Abstract: | This study was to molecular identify Prunus necrotic ringspot virus (PNRSV) occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV. An expected cDNA fragment of about 450 bp was amplified from lily, cloned into pGEM-T-easy vector, sequenced, and shared 84.5-99.1% homology with the 27 PNRSV isolates reported previously at the nucleotide level, indicating that lily is a new natural host of PNRSV. |
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| Keywords: | lily Prunus necrotic ringspot virus RT-PCR identification |
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