绵阳地区山羊粪便中捻转血矛线虫的分子鉴定

为鉴定绵阳地区山羊粪便中疑似血矛属线虫虫卵的虫种,本研究提取绵阳地区三个县山羊粪便中疑似血矛属线虫的虫卵DNA 60份,采用PCR技术扩增其核糖体第二内转录间隔区(rDNA ITS-2)基因序列,并与GenBank上公布的捻转血矛线虫同源序列进行比较分析。结果显示:60份疑似血矛属线虫的虫卵DNA样品均得到191bp的ITS-2片段;60条ITS-2序列共形成15条不同的序列,含有17个单变异位点,与GenBank中多株捻转血矛线虫的同源性达95.8%~100%;ML系统发育树显示,本研究得到的15种类型ITS-2序列与已报道的捻转血矛线虫(Haemonchus contortus)聚类形成一个分支。综上可得,60份来自山羊的血矛属线虫均为捻转血矛线虫。

Molecular identification of Haemonchus contortus in faeces of goats from Mianyang area

To identify the suspected Haemonchus sp.egg specimens in faeces of goats from three Counties in Mianyang area,60 egg specimens were used for DNA extraction,and the internal transcribed spacers(rDNA ITS-2)were amplified and sequenced,then compared with other homologous sequences of H.contortus available from GenBank.The results showed that ITS-2 sequences were successfully amplified from 60 egg specimens,and the length of ITS-2 sequence alignments covered 191 bp.The 60 sequences were classified into 15 different sequences,containing 17 variable sites.The similarity of ITS-2 sequences varied from 95.8 to 100.0%among our sequences and other H.contortus sequences from GenBank.The to⁃pologies in ML phylogenetic tree showed that the 15 different ITS-2 sequences obtained in this study and other reported H.contortus clustered in the same clade.These results indicated that all 60 specimens from goats in Mianyang area were Haemonchus contortus.