水稻稻曲病菌药剂室内生物测定和筛选

目的]筛选出有效控制水稻稻曲病菌(Ustilaginoidea virens)的杀菌剂。方法]采用菌丝生长速率测定法,分别测定烯唑醇、丙环唑、咪鲜胺杀菌剂对稻曲病病菌的毒力,并采用最小二乘法求得其毒力回归方程。结果]烯唑醇对稻曲病菌的毒力方程为:Y=0.331 4X+5.946 5(R2=0.941 8),计算得烯唑醇对稻曲病菌的EC50=0.06μg/ml;丙环唑对稻曲病菌的毒力方程为:Y=0.489X+6.175 1(R2=0.982 4),计算的得丙环唑对稻曲病菌EC50=0.09μg/ml。咪鲜胺对稻曲病菌的毒力方程为:Y=0.116 3X+5.032 1(R2=0.99),计算得咪鲜胺对稻曲病菌的EC50=0.76μg/ml。结论]烯唑醇对稻曲病菌毒力最高,丙环唑次之,咪鲜胺最小。

Indoor Bioassay and Screening of Medicaments against Ustilaginoidea virens in Rice

Objective] The research aimed to screen out the bactericides that could control Ustilaginoidea virens in rice effectively.Method] The toxicities of bactericides including diniconazole,propiconazole and prochloraz to U.virens were determined respectively by the determination method of mycelium growth rate.And the regression equations of their toxicities were calculated by using least square method.Result] The toxicity equation of diniconazole to U.virens was Y=0.331 4X+5.946 5(R2=0.941 8)and the EC50 value of diniconazole to U.virens was calculated as 0.06 μg/ml.The toxicity equation of propiconazole to U.virens was Y=0.489X+6.175 1(R2=0.982 4) and the EC50 value of propiconazole to U.virens was calculated as 0.09 μg/ml.The toxicity equation of prochloraz to U.virens was Y=0.116 3X+5.032 1(R2=0.99) and the EC50 value of prochloraz to U.virens was calculated as 0.76 μg/ml.Conclusion] The toxicity of diniconazole to U.virens was highest,followed by propiconazole and that of prochloraz was lowest.