| 蟾蜍壶菌病病原遗传分化研究 |
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| 引用本文： | 曾朝辉,白世卓,朱蕴绮,王晓龙.蟾蜍壶菌病病原遗传分化研究[J].经济动物学报,2011(3):160-163. |
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| 作者姓名： | 曾朝辉 白世卓 朱蕴绮 王晓龙 |
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| 作者单位： | 北京自然博物馆;东北林业大学野生动物资源学院;东北林业大学保护医学与生态安全研究中心; |
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| 基金项目： | 北京市科学技术研究院“萌芽后续”人才培养计划资助项目(2010-07) |
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| 摘 要： | 壶菌病为近年发现的两栖类动物重要传染病，对野生和养殖种群危害极大，为确定药用经济动物蟾蜍历史上壶菌病感染情况，提高养殖蟾蜍疾病防治水平，选取某博物馆馆藏采集于四川的蟾蜍标本32只，利用Taqman—MGB荧光探针定量PCR技术进行壶菌检测，并对定量PCR产物克隆、测序，通过序列比对和系统发育分析判定其来源。最终得到定量...
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| 关 键 词： | 蟾蜍 馆藏标本 壶菌 实时PCR 系统发育分析 |
Genetic Differentiation of the Pathogen of Batrachochytrium dendrobatidis in Toads |
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| ZENG Zhao-hui,BAI Shi-zhuo,ZHU Yun-qi,WANG Xiao-long.Genetic Differentiation of the Pathogen of Batrachochytrium dendrobatidis in Toads[J].Journal of Economic Animal,2011(3):160-163. |
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| Authors: | ZENG Zhao-hui BAI Shi-zhuo ZHU Yun-qi WANG Xiao-long |
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| Institution: | ZENG Zhao-hui1,BAI Shi-zhuo2,3,ZHU Yun-qi2,WANG Xiao-long2,3(1.Beijing Museum of Natural History,Beijing 100050,China,2.Wildlife Resource College,Northeast Forestry University,Harbin 150040,3.Center of Conservation Medicine and Ecological Safety,China) |
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| Abstract: | The chytridiomycosis is a significant emerging disease of amphibians in recent years which seriuosly does harm to the wild and breeding population.To ascertain the condition of the Batrachochytrium dendrobatidis infection history in the population of toad,the medicinal economic animal,and enhance the prevention for this disease,32 toad specimen collected from Sichwan keeping in a museum were assayed by Taqman-MGB fluorescence probe quantitative polymerase chain reaction to detect Batrachochytrium dendrobati... |
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| Keywords: | toad specimens collection Batrachochytrium dendrobatidis real-time PCR phylogenetic analysis |
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