Tomato: a crop species amenable to improvement by cellular and molecular methods |
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Authors: | Jacoues Hille Maarten Koornneef M. S. Ramanna Pim Zabel |
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Affiliation: | (1) Department of Genetics, Free University, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands;(2) Department of Genetics, Agricultural University, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands;(3) Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands;(4) Department of Molecular Biology, Agricultural University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands |
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Abstract: | Summary Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures.In view of the current data, the isolation of useful mutants at the cellular level probably will be of limited value in the genetic improvement of tomato. Protoplast fusion may lead to novel combinations of organelle and nuclear DNA (cybrids), whereas this technique also provides a means of introducing genetic information from alien species into tomato. Important developments have come from molecular approaches. Following the construction of an RFLP map, these RFLP markers can be used in tomato to tag quantitative traits bred in from related species. Both RFLP's and transposons are in the process of being used to clone desired genes for which no gene products are known. Cloned genes can be introduced and potentially improve specific properties of tomato especially those controlled by single genes. Recent results suggest that, in principle, phenotypic mutants can be created for cloned and characterized genes and will prove their value in further improving the cultivated tomato. |
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Keywords: | Lycopersicon esculentum tomato genetic transformation protoplast fusion restriction fragment length polymorphisms (RFLP's) transposons |
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