| 番鸭小鹅瘟病直接荧光诊断试剂的研制 |
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| 引用本文： | 朱小丽,陈少莺,程晓霞,林锋强,陈仕龙,王劭,李兆龙,王燕玲. 番鸭小鹅瘟病直接荧光诊断试剂的研制[J]. 畜牧兽医杂志, 2012, 31(4): 1-3,7 |
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| 作者姓名： | 朱小丽 陈少莺 程晓霞 林锋强 陈仕龙 王劭 李兆龙 王燕玲 |
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| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福州350013 福建省畜禽疫病防治工程技术研究中心
2. 福建省农业科学院畜牧兽医研究所,福州,350013 |
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| 基金项目： | 福建省农业科学院创新团队，省公益类项目，国家公益性行业(农业)科研专项子课题 |
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| 摘 要： | 将抗番鸭GPV单抗腹水采用透析法标记异硫氰酸荧光素（FITC），制备成抗GPV荧光抗体，研制检测GPV抗原的直接免疫荧光诊断方法。结果显示GPV荧光抗体仅与GPV阳性的组织切片或细胞呈现特异性荧光，与番鸭细小病毒（MPV）、番鸭呼肠孤病毒（MDRV）、鸭副粘病毒（DPMV）、鸭病毒性肝炎病毒（DHV）和正常番鸭组织切片不反应；与间接荧光方法的符合率为92．9％。表明GPV荧光抗体具有较好的特异性、敏感性和准确性，可用于临床快速诊断番鸭小鹅瘟病。
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| 关 键 词： | 番鸭小鹅瘟 荧光抗体 直接免疫荧光 |
Praparation for Direct Fluorescence Diagnosis Reagent on Muscovy Duck Gosling Plague |
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| ZHU Xiao-li,CHEN Shao-ying,CHENG Xiao-xia,LIN Feng-qiang,CHEN Shi-long,WANG Shao,LI Zhao-long,WANG Yan-ling. Praparation for Direct Fluorescence Diagnosis Reagent on Muscovy Duck Gosling Plague[J]. Journal of Animal Science and Veterinary Medicine, 2012, 31(4): 1-3,7 |
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| Authors: | ZHU Xiao-li CHEN Shao-ying CHENG Xiao-xia LIN Feng-qiang CHEN Shi-long WANG Shao LI Zhao-long WANG Yan-ling |
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| Affiliation: | 1(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences,Fuzhou Fujian 350013,China; 2.Fujian Animal Diseases Control Technology Development Center) |
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| Abstract: | Monoclonal antibody against goose parvovirus derived from muscovy duck was used to mark with fluorescein isothiocyanate by dialysis to manufacture fluorescent antibody in orger to prepare direct fluorescence assay(DFA) for gosling plague virus.The results showed that GPV fluorescent antibody only reacted with GPV-positive-tissue slice or cell to present specific fluorescent,but not react with muscovy duck parvovirus(MPV),muscovy duck reovirus(MDRV)、duck paramyxoviruses(DPMV),duck hepatitis virus(DHV) and normal tissue.The coincidence of DFA and with indirect fluorescence assay(IFA) was 92.9% for detecting GPV.The results above indicated that GPV fluorescent antibody had good specificity,sensitivity and accuracy so as to diagnose muscovy duck gosling plague. |
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| Keywords: | muscovy duck gosling plague fluorescence antibody firect immuno-fluorescence assay |
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