| 中华结缕草组织培养与植株再生 |
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| 引用本文： | 杜雪玲,张振霞,刘萍,龚春水,符义坤,杨中艺. 中华结缕草组织培养与植株再生[J]. 草业学报, 2006, 15(4): 88-93 |
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| 作者姓名： | 杜雪玲 张振霞 刘萍 龚春水 符义坤 杨中艺 |
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| 作者单位： | 安徽淮北煤炭师范学院生物系,安徽,淮北,235000;韩山师范学院生物系,广东,潮州,521041;中山大学生命科学院,广东,广州,510275;甘肃农业大学草业学院,甘肃,兰州,730070 |
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| 基金项目： | 国家转基因植物研究与产业化专项(J2002-B-006)资助 |
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| 摘 要： | 以中华结缕草成熟胚为外植体,通过胚性愈伤组织的发生进行植株再生。结果表明,中华结缕草成熟胚在MS基本培养基附加6~7 mg/L 2,4-D和0.05 mg/L 6-BA的出愈能力很强,但初生愈伤组织呈无定型棉絮状,不能再生成株。采用不同的糖类和固化剂以及一定比例的生长素与细胞分裂素的方法,对初生愈伤组织进行继代培养,诱导出致密颗粒状的、具有高度再生能力的胚性愈伤组织,此类胚性愈伤组织在最佳分化培养基MS 1.0mg/L NAA 1.0 mg/L KT 3.00 mg/L 6-BA上有较高再生频率,生根培养基采用1/2 MS。
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| 关 键 词： | 中华结缕草 愈伤组织 植株再生 |
| 文章编号： | 1004-5759（2006）04-0088-06 |
| 收稿时间： | 2004-08-23 |
| 修稿时间： | 2004-08-23 |
Tissue culture and plant regeneration of Zoysia siasinica |
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| DU Xue-ling,ZHANG Zhen-xia,LIU Ping,GONG Chun-shui,FU Yi-kun,YANG Zhong-yi. Tissue culture and plant regeneration of Zoysia siasinica[J]. Acta Prataculturae Sinica, 2006, 15(4): 88-93 |
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| Authors: | DU Xue-ling ZHANG Zhen-xia LIU Ping GONG Chun-shui FU Yi-kun YANG Zhong-yi |
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| Affiliation: | 1. Department of Biology, Huaibei Coal Industry Teachers College, Huaibei 235000, China; 2. Biology,Hanshan Normal University, Chaozhou 521041, China; 3. College of Life Science, Zhongshan University, Guangzhou 510275, China; 4. College of Grassland Science,Gansu Agricutural University, Lanzhou 730070, China |
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| Abstract: | A tissue culture system for plant regeneration of Zoysia siasinica Hance grass was developed.Using mature seeds as experimental material, explant callus was produced in the dark on MS containing 100 mg/L Linostial 5 mg/L 2,4-D 0.05 mg/L6-BA,30 mg/L sucrose and 3.4 g/L phytagel.The initial callus appeared sponge-like,soft,and irregular,and no plants could be regenerated from it.High regeneration ability embryogenic calluses were induced after subculturing with 2 mg/L ABA.The differentiation frequency of callus subcultured for 80 days was up to 20.83% in MS with 1.0 mg/L NAA,1.0 mg/L KT and 3.00 mg/L 6-BA MS.The rooting medium was 1/2 MS. |
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| Keywords: | Zoysia siasinica callustissue culture regeneration |
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