一个与双孢蘑菇子实体品质相关的DNA片段的克隆

利用20个十碱基随机引物，在基因组水平上对双孢蘑菇（Agaricus bisporus）的优质低产型（G型）及低抽高产型（H型）菌析进行了RAPD差异显示，获得了一个与双孢蘑菇子实体品质相关的大小约为2000bp的差异DNA片段，将其克隆，并应用点杂交及RFLP技术对其区分两类菌株的有效性进行了检验。这为进一步建立和的特异性探针或PCR引物，从分子水平上提高双孢蘑菇菌种性状早期预测的准确性奠定了基础。

Cloning of a DNA Fragment Correlated to the Quality of Agaricus bisporus Fruitbody

With 20 ten-mers random primers, the genomic differences between two types of Agaricus bisporus strain-good quality with low yield (G type) vs. poor quality with high yield (H type), were displayed by Random Amplified Polymorphic DNA (RAPD) technique. A 2000bp specific DNA fragment correlated to the quality of Agaricus bisporus fruitbody was obtained and cloned. Then dot hybridization and RFLP technique were used to determine the effectiveness of distinguishing two types of A. bisporus strains. And it was considered that our study had laid a foundation for further establishing a specific probe or PCR primer and for improving the accuracy of early predicting the characteristics of A. bisporus strains at molecular level.