Euphresco Sendo: An international laboratory comparison study of molecular tests for Synchytrium endobioticum detection and identification |
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Authors: | Bart van de Vossenberg Marcel Westenberg Ian Adams Olga Afanasenko Ani Besheva Margriet Boerma James Choiseul Toos Dekker Kerstin Flath Marga van Gent-Pelzer Kurt Heungens Anatolii Karelov Ilona Kibildiene Jaroslaw Przetakiewicz Alexandra Schlenzig Vera Yakovleva Gerard van Leeuwen |
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Affiliation: | 1.Dutch National Plant Protection Organization,National Reference Centre,Wageningen,The Netherlands;2.Fera Science Ltd.,York,UK;3.All Russian Research Institute for Plant Protection,Saint Petersburg,Russia;4.Central laboratory for plant quarantine,Sofia,Bulgaria;5.Hilbrands Laboratorium BV,Wijster,The Netherlands;6.Department of Agriculture, Food and the Marine,Backweston Campus,Celbridge,Ireland;7.NAK,Emmeloord,the Netherlands;8.Julius Kühn-Institut,Kleinmachnow,Germany;9.Wageningen Plant Research,BU Biointeractions & Plant Health,Wageningen,The Netherlands;10.Institute for Agricultural and Fisheries Research,Merelbeke,Belgium;11.Institute of Plant Protection,Kiev,Ukraine;12.The State Plant Service, Phytosanitary Research Laboratory (Division),Ministry of Agriculture,Vilnius,Lithuania;13.Plant Breeding and Acclimatization Institute,National Research Institute,Blonie,Poland;14.Science and Advice for Scottish Agriculture,Edinburgh,UK;15.All-Russian Plant Quarantine Center,Ramenskoe region,Russia |
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Abstract: | An international test performance study (TPS) was organised to generate validation data for three molecular Synchytrium endobioticum tests: van den Boogert et al. (European Journal of Plant Pathology 113, 47–57, 2005), and van Gent-Pelzer et al. (European Journal of Plant Pathology, 126, 129-133, 2010) for the detection of S. endobioticum, and the pathotype 1(D1) identification test described by Bonants et al. (European Journal of Plant Pathology, 143, 495-506, 2015). Two TPS rounds were organised focussing on different test matrices, i.e. round 1: warted potato tissue, and round 2: resting spore suspensions. When using the tests for detection and identification of S. endobioticum in warted potato tissue, no significant differences were observed for diagnostic sensitivity, diagnostic specificity, overall accuracy, analytical sensitivity and robustness. When using the tests for detection and identification of S. endobioticum in resting spore suspensions, the van den Boogert and van Gent-Pelzer tests significantly outperform the Bonants test for diagnostic sensitivity and diagnostic specificity. For overall accuracy and analytical sensitivity, the van Gent-Pelzer significantly outperforms the van den Boogert and Bonants tests and is regarded as the test of choice when identifying S. endobioticum from resting spores. Tests regarded fit for purpose for routine testing of wart material and resting spore suspensions are proposed for the update of EPPO standard PM7/28(1) Synchytrium endobioticum. |
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