Characterization of Citrus tristeza virus isolates in northern Iran |
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Authors: | Ali Barzegar Haleh Hashemi Sohi Heshmatollah Rahimian |
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Institution: | (1) Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran;(2) Plant Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, 15 Shahid Shafiee Alley, Ghods Street, Enghelab Avenue, Tehran, Iran;(3) College of Agriculture, Mazandaran University, Khazarabad Street, Sari, Iran |
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Abstract: | The biological and molecular properties of four Citrus tristeza virus (CTV) isolates isolated from infected Satsuma trees imported from Japan, and growing in citrus groves in northern Iran (Mahdasht
orchards, Mazandaran Province), were investigated. CTV-infected samples were collected from sweet orange trees and grafted
onto Alemow (Citrus macrophylla Wester) seedlings. On indicator plants, these isolates produced various symptoms including vein clearing and stem pitting
on Mexican lime, Alemow, and Citrus hystrix, and yellowing and stunting on sour orange and grapefruit seedlings. Citrus samples were also surveyed for CTV using serological
tests. The coat protein (CP) gene of these isolates was amplified using specific primers, yielding an amplicon of 672 bp for
all isolates. Sequence analysis showed 98%–99% sequence homology of Iranian isolates with the Californian CTV severe stem-pitting
isolate SY568 and 97%–98% homology with the Japanese seedling yellows isolate NUagA. The Iranian isolates were compared by
restriction fragment length polymorphism (RFLP) analysis of the CP amplicon for further classification. |
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Keywords: | Citrus tristeza virus Coat protein gene Iranian isolates Nucleotide sequencing RFLP Biological properties |
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