| QuEChERS-液相色谱-串联质谱法测定鲤肝脏中氟虫腈及其代谢物残留 |
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| 引用本文： | 马丽莎,单奇,谢文平,尹怡,刘书贵,李丽春,赵成,郑光明. QuEChERS-液相色谱-串联质谱法测定鲤肝脏中氟虫腈及其代谢物残留[J]. 中国渔业质量与标准, 2021, 0(2): 18-24 |
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| 作者姓名： | 马丽莎 单奇 谢文平 尹怡 刘书贵 李丽春 赵成 郑光明 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所 |
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| 基金项目： | 国家水产品质量安全风险评估-稻渔综合种养水产品安全性评估(GJFP201800902);海南省热带海洋生物技术重点实验室开放基金项目(LTMB201904);中国-东盟海上合作基金(CAMC-2018F)。 |
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| 摘 要： | 以QuEChERS作为样品前处理方法,结合高效液相色谱-串联质谱(HPLC-MS/MS)检测技术,建立了鲤(Cyprinus carpio)肝脏中氟虫腈(fipronil)及代谢物氟甲腈(fipronil desulfinyl)、氟虫腈亚砜(fipronil sulfide)和氟虫腈砜(fipronil sulfone...
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| 关 键 词： | 液相色谱-串联质谱(LC-MS/MS) QuEChERS 鲤肝脏 氟虫腈及其代谢物 |
Determination of Fipronil and Its Metabolites in Liver Tissue of Cyprinus carpio by QuEChERS Combined with Liquid Chromatography-Tandem Mass Spectrometry |
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| MA Lisha,SHAN Qi,XIE Wenping,YIN Yi,LIU Shugui,LI Lichun,ZHAO Cheng,ZHENG Guangming. Determination of Fipronil and Its Metabolites in Liver Tissue of Cyprinus carpio by QuEChERS Combined with Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Fishery Quality and Standards, 2021, 0(2): 18-24 |
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| Authors: | MA Lisha SHAN Qi XIE Wenping YIN Yi LIU Shugui LI Lichun ZHAO Cheng ZHENG Guangming |
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| Affiliation: | (Pear River Fisheries Research Institute,Chinese Academy of Fishery Sciences, Minisitry of Agriculture Laboratory of Quality & Safety Risk Assessment for Aquatic Product(Guangzhou),Key Laboratory of Recreational Fisheries Research, Ministry of Agriculture, Guangzhou 510380,China) |
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| Abstract: | A determination method of fipronil and its metabolites(fipronil desulfinyl,fipronil sulfide and fipronil sulfone)in liver tissue of Cyprinus carpio was developed by quick,easy,cheap,effective,rugged,and safe(QuEChERS)extraction coupled with high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS).Samples were extracted with 0.1%formic acid acetonitrile,and purified by neutral alumina,PSA and GCB.Then,fipronil and its metabolites were detected by using LC-MS/MS.The analytes were quantified with external standard using the matrix-matched standard calibration curve method.The results showed that the average spiked recoveries of fiproni and its metabolites in liver tissue of fish were 87.3%~109.2%with relative standard deviations(RSDs)of 1.3%~12.0%.Good linearities were obtained in the range of 0.4~100.0 ng/mL,and the correlation coefficients were greater than 0.997,the LODs were 2.0μg/kg,and the LOQs were 5.0μg/kg.The method was simple,rapid and sensitive.And the preparation for a single sample was within 20min.The developed method is suitable for studying the metabolism and residue elimination of fipronil in fish liver. |
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| Keywords: | liquid chromatography-tandem mass spectrometry(LC-MS/MS) QuEChERS carp liver fipronil and its metabolites |
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