| 抗传染性法氏囊病病毒单克隆抗体的研究Ⅰ．抗传染性法氏囊病病毒（CJ801株）单克隆抗体杂交瘤细胞株的建立 |
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| 引用本文： | 张曼夫,江青艳. 抗传染性法氏囊病病毒单克隆抗体的研究Ⅰ．抗传染性法氏囊病病毒（CJ801株）单克隆抗体杂交瘤细胞株的建立[J]. 畜牧兽医学报, 1996, 0(4) |
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| 作者姓名： | 张曼夫 江青艳 |
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| 作者单位： | 北京农业大学生物学院 |
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| 摘 要： | 应用杂交瘤技术获得了３株抗传染性法氏囊病病毒（ＣＪ８０１株）的单克隆抗体。３株单抗与鸡新城疫病毒、鸡传染性支气管炎病毒、鸡传染性喉气管炎病毒、鸡马立克氏病病毒均无交叉反应。ＣｏｍｐｅｔｉｔｉｖｅＥＬＩＳＡ表明、３株单抗所对应的抗原位点互不重叠。Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ试验表明、３株单抗所对应的抗原位点都位于ＶＰ２ｂ上。
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| 关 键 词： | 传染性法氏囊病病毒，单克隆抗体 |
STUDIES ON MONOCLONAL ANTIBODIES AGAINST INFECTIOUS BURSAL DISEASE VIRUS I.THE ACQUIBEMENT OF HYBRIDOMAS SECRETING MONOCLONAL ANTIBODIES AGAINST INFECTIOUS BURSAL DISEASE VIRUS(CJ801 STBAIN) |
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| Zhang Manfu,Jiang Qingyan. STUDIES ON MONOCLONAL ANTIBODIES AGAINST INFECTIOUS BURSAL DISEASE VIRUS I.THE ACQUIBEMENT OF HYBRIDOMAS SECRETING MONOCLONAL ANTIBODIES AGAINST INFECTIOUS BURSAL DISEASE VIRUS(CJ801 STBAIN)[J]. Chinese Journal of Animal and Veterinary Sciences, 1996, 0(4) |
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| Authors: | Zhang Manfu Jiang Qingyan |
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| Abstract: | Three monoclonal antibodies(McAbs)were produced against CJ801 strain of infec-tious bursal disease virus(IBDV)using hybridoma technique. No crossreaction wasobserved between the McAbs and Newcastle disease virus,infectious bronchitis virus,infcctious laryngotracheitis virus and Marek's disease virus.Results of competitivecnzyme-linked immunosorbent assay(ELISA)showed that the epitopes defined by McAbAB7,BG9,BE10 were not overlapping. Western blotting indicated that the epitopes reco-gnized by McAb AB7,BG9,BE10 wereall present on VP2b of IBDV. |
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| Keywords: | infectious bursal disease virus MonocJonal antibodies |
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