Indica-japonica differentiation in Chinese rice landraces |
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Authors: | Wen-Bing Chen Yo-Ichiro Sato Ikuo Nakamura Hirokazu Nakai |
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Institution: | (1) The United Graduate School of Agricultural Science, Gifu Univ., Yanagido, 501-11 Gifu City, Japan;(2) National Institute of Genetics, 411 Mishima City, Japan;(3) Iwate Biotechnology, Research Center, 024 Kitakami City, Japan;(4) Faculty of Agriculture, Shizuoka University, 422 Shizuoka City, Japan |
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Abstract: | Summary Ninety Chinese rice landraces were examined with special reference to the indica-japonica differentiation in terms of traditional criteria, isozyme analysis and PCR analysis of the chloroplast DNA (cpDNA). Cultivars were separated into indica and japonica defined by a discriminant function (Z) based on key characters, as well as by isozyme genotypes. Most indica landraces had chloroplast DNAs with a deletion at the Pst-12 fragment, while most japonica landraces had cpDNAs without the deletion. Two traditionally recognized varietal groups in China, keng and hsien, corresponded largely to the respective japonica and indica revealed in our study. The results obtained in this study showed good agreement for classification of indica and japonica types by the three methods: discriminant analysis by Z value, isozyme analysis, and PCR analysis for cpDNA. |
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Keywords: | Oryza sativa landraces varietal differentiation hsien and keng indica and japonica isozymes chloroplast DNA (cpDNA) Polymerase Chain Reaction (PCR) |
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