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| 番茄红素环化酶基因片段克隆及反义表达载体构建 | |
| 引用本文： | 李莉,侯丽霞,施木田,吕鑫,何启伟. 番茄红素环化酶基因片段克隆及反义表达载体构建[J]. 山东农业科学, 2009, 0(1): 1-3 |
| 作者姓名： | 李莉 侯丽霞 施木田 吕鑫 何启伟 |
| 作者单位： | 1. 福建农林大学园艺学院,福建福州,350002 2. 山东省农业科学院蔬菜研究所,山东济南,250100 3. 吉林农业大学园艺学院,吉林长春,130118 |
| 基金项目： | 山东省农业科学院博士科研启动基金 |
| 摘 要： | 根据已知的番茄红素环化酶基因，即13环化酶基因和s环化酶基因的保守序列设计特异性引物。提取番茄叶片的RNA，经RT—PCR反应，扩增出723bp和569bp的目的片段，将它们分别连接到pEASY—T1 Cloning Vector上，测序鉴定其正确性。克隆到载体上的13环化酶基因用BamHI和SmaI双酶切，将基因反向插入PROK2表达载体上，构建PROK2-LYCb反义表达载体。同样连接到克隆载体上的s环化酶基因用BamHI和XbaI双酶切，将基因反向插入PROK2表达载体上，构建PROK2-LYCe反义表达载体。 |
| 关 键 词： | 番茄红素 β环化酶基因 ε环化酶基因 克隆 反义表达载体 |
Cloning and Construction of Antisense Expression Vectors of Lycopene Cyclase Genes |
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| LI Li,HOU Li-xia,SHI Mu-tian,Lü Xin,HE Qi-wei. Cloning and Construction of Antisense Expression Vectors of Lycopene Cyclase Genes[J]. Shandong Agricultural Sciences, 2009, 0(1): 1-3 | |
| Authors: | LI Li HOU Li-xia SHI Mu-tian Lü Xin HE Qi-wei |
| Affiliation: | 1.College of Horticulture;Fujian Agriculture and Forestry University;Fuzhou 350002;China;2.Institute of Vegetable;Shandong Academy of Agricultural Sciences;Jinan 250100;3.College of Horticulture;Jilin Agricultural University;Changchun 130118;China |
| Abstract: | Based upon the known lycopene cyclase genes β and ε,specific primers were designed.The RNA extracted from tomato leaves was used for RT-PCR,then the 723 bp and 569 bp gene fragments were amplified and were subcloned into pEASY-T1 Cloning Vectors for sequencing.LYCb gene was digested by BamHⅠ and SmaⅠ and inserted into PROK2 expressing vector to construct the antisense vector PROK2-LYCb.In the same way,LYCe gene was digested by BamHⅠ and XbaⅠand inserted into PROK2 expressing vector to construct the antisense vector PROK2-LYCe. |
| Keywords: | Lycopene LYCb LYCe Cloning Antisense expression vector |
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