Effects of glandular trichomes on the development of Phytophthora infestans infection in potato (S. tuberosum) |
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Authors: | Antonia Lai Valeria Cianciolo Salvatore Chiavarini Andrea Sonnino |
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Institution: | (1) C.R. Casaccia, Dipartimento Innovazione, Divisione Biotecnologie e Agricoltura, ENEA, Via Anguillarese 301, 00060 Rome, Italy;(2) C.R. Casaccia, Dipartimento Ambiente, ENEA, Via Anguillarese 301, 00060 Rome, Italy |
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Abstract: | S. berthaulltii Hawkes, a wild potato species, possesses two types of glandular trichomes on its leaf surface: types A and B wich confer
resistance to insects. Preliminary results indicated that the presence of glandular trichomes also confers resistance to Phytophthora infestans, the causal agent of late blight. In this paper we report the evaluation of trichome characteristics (density and exudate
activities) performed on 8 lines of potato, including the control varieties Desirée and Tropicana, 5 hybrid lines derived
from intercrosses and backcrosses between S. tuberosum and S. berthaultii and one accession of S. berthaultii. Whole plants of the 8 genotypes were inoculated with a spore suspension of Phytophthora infestans in order to evaluate their resistance to late blight. The resultant disease incidence correlated negatively with type A trichome
density and with polyphenol-oxidase (PPO) activity of type A trichome glands. The removal of type B trichomes exudate by leaf
dipping in ethanol did not influence the disease development after artificial inoculation of detached leaves. Type B trichome
exudate was extracted and the fraction corresponding to Fatty Acids Sucrose Esters (FASE) was recovered after Thin Layer Chromatography
and tested on the fungal mycelium in vitro. The FASE exhibited a slight inhibitory effect on mycelium growth. The results of the experiments are discussed, and several
hypotheses regarding the possible role of types A and B trichomes are formulated.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | bioassay exudates inoculation late blight resistance S berthaultii |
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