| 副鸡嗜血杆菌PCR检测方法的建立 |
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| 引用本文： | 张欢,侯佳蕾,张彦红,罗晶璐,任涛.副鸡嗜血杆菌PCR检测方法的建立[J].广东畜牧兽医科技,2009,34(1):28-30. |
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| 作者姓名： | 张欢 侯佳蕾 张彦红 罗晶璐 任涛 |
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| 作者单位： | 华南农业大学兽医学院,农业部养禽与禽病防治重点开放实验室,广东,广州,510642 |
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| 摘 要： | 参照GenBank中已发表的副鸡嗜血杆菌血凝素（HA）基因设计合成了一对引物，预计扩增片段大小约为412bp。利用这对引物，通过对PCR反应体系和反应条件的优化，建立了针对副鸡嗜血杆菌的PCR检测方法。结果表明，该方法敏感性较高，最低可检出1．7×10^4CFU／mL的副鸡嗜血杆菌，对副猪嗜血杆菌、巴氏杆菌、大肠杆菌、沙门氏菌在相同反应条件下未扩增出任何片段，说明该方法具有较好的特异性。
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| 关 键 词： | 副鸡嗜血杆菌 PCR检测 |
Establishment of a PCR Detection Method for Haemophilus Paragallinarum |
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| Zhang Huan,Hou Jialei,Zhang Yanhong,Luo Jinglu,Ren Tao.Establishment of a PCR Detection Method for Haemophilus Paragallinarum[J].Guangdong Journal of Animal and Veterinary Science,2009,34(1):28-30. |
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| Authors: | Zhang Huan Hou Jialei Zhang Yanhong Luo Jinglu Ren Tao |
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| Institution: | (Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China) |
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| Abstract: | A PCR detection method for Haemophilus paragallinarum was established with a pair of specific primers against the conserved regions of HA gene. The PCR products were 412 bp long. This method was sensitive and specific. More than 1.7×10^4CFU/ml Haemophilus paragallinarum strains could be detected and no reactions was found for Avian pasteurella strains, Haemophilus parasuis strains, Salmonella strains and Escherichia coli strains. |
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| Keywords: | Haemophilus paragallinarum PCR detection method |
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