| 用于PCR分析的小麦种子DNA微量快速提取 |
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| 引用本文： | 董建力,张增艳,王敬东,惠红霞. 用于PCR分析的小麦种子DNA微量快速提取[J]. 种子, 2007, 26(5): 10-12 |
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| 作者姓名： | 董建力 张增艳 王敬东 惠红霞 |
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| 作者单位： | 1. 宁夏农林科学院农业生物技术研究中心,银川,750002
2. 中国农科院作物科学研究所,北京,100081 |
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| 摘 要： | 探索了不用液氮从小麦单粒、半粒有胚和半粒无胚中提取DNA的方法。结果表明，无论是单粒、半粒有胚和半粒无胚都能得到比较理想的DNA，DNA样品经SCAR-PCR特异标记检测，都能扩增出两条完全相同的特异务带。用无胚的半粒进行分子标记检测特别适合于对大批量早代材料目的基因的筛选。
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| 关 键 词： | 小麦 籽粒 |
| 文章编号： | 1001-4705（2007）05-0010-03 |
| 修稿时间： | 2007-01-28 |
Rapid Extraction of DNA from Wheat Seed for PCR Analysis |
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| DONG Jian-li,ZHANG Zeng-yan,WANG Jin-dong,HUI Hong-xia. Rapid Extraction of DNA from Wheat Seed for PCR Analysis[J]. Seed, 2007, 26(5): 10-12 |
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| Authors: | DONG Jian-li ZHANG Zeng-yan WANG Jin-dong HUI Hong-xia |
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| Affiliation: | 1. Agricultural Bio-Technology Center of Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750005, China ; 2. Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Science, Beijing 100081, China |
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| Abstract: | This paper mainly studies the method of extracting DNA from the single seed, 1/2 seed with embryo and 1/2 seed without embryo of wheat without liquid N2. The result indicates the high quality DNAs can be obtained from above different partitions and the same SCAR-PCR products can be observed using them as templet. Particularly, DNA extracted from 1/2 seed without embryo of wheat is suited to screen target DNAs from earlier offspring of wheat by SCAR-PCR. |
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| Keywords: | DNA PCR |
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