大豆铁结合蛋白基因的克隆及其推定蛋白结构分析

根据GeneBank数据库中大豆类铁结合蛋白序列设计引物,通过反转录方法从豫豆8号中克隆到cDNA片段,测序结果表明,该基因cDNA全长752 bp,编码250氨基酸.利用DNAMAN软件进行序列比对分析发现,该cDNA的氨基酸序列与大豆(M64337.1)铁结合蛋白同源性高达98%以上.运用生物信息学软件及相关网站对其功能位点、结构功能域分析,结果显示,该基因具有1个FER-RITIN-LIKE功能域、2个铁结合蛋白基因家族铁结合区域的信号序列、6个磷酸化位点等重要功能位点.

Cloning of the iron-binding protein gene from soybean and analysis of its putative protein structure

Primers were designed according to beans' ferritin sequence registered in GeneBank, Yu-dou 8 was used as experimental material and RT - PCR was employed in order to clone ferritin gene. A full-length cDNA, 752 bp, 250 amino acids encoded, was cloned. Sequence alignment revealed that there was above 98% identity between soybean (M64337. 1) ferritin and current sequence. The puta-tive protein structure features were analyzed by bioinformatics softwares and relevant web sites. Results showed that there are one FER-RITIN-LIKE function domain, two iron-binding sites, six phosphoryla-tion sites, and other important sites in the protein.