高效液相色谱法测定水稻上的三环唑

应用高效液相色谱法-紫外检测器(HPLC-UVD)对水稻植株、糙米、田水样品及土壤中的三环唑进行检测,三环唑在3种添加水平下:1田水样品添加水平为0.008 8~0.88mg/kg时,回收率为83.53%~108.0%,相对标准偏差(RSD)为0.832%~7.24%;2土壤样品添加水平为0.017 6~1.76mg/kg时,回收率为78.63%~94.17%,相对标准偏差(RSD)为2.60%~4.43%;3植株和糙米添加水平为0.035 2~3.52mg/kg时,回收率分别为72.01%~97.42%,81.78%~97.06%,相对标准偏差(RSD)分别为3.52%~5.91%,1.69%~10.1%。该方法的浓度范围为0.352~35.2μg/mL时,最低检出限为4.2×10-10g,最低检出浓度为0.008 8mg/kg。本方法操作简单、快速、定量准确,可有效适用于三环唑在水稻上的残留检测。

Determination of Tricyclazole in Rice by HPLC

Tricyclazole was detected in paddy,brown rice,water,and soil with high performance liquid chromatography （HPLC） equipped with ultraviolet （UV） detector.Tricyclazole wasadded in three levels：①when the level of water was 0.008 8～0.88mg/kg,the average recovery was 83.53％～108.0％,and the relative standard deviation （RSD） was 0.832％～7.24％;②when the level of soil was 0.017 6～1.76mg/kg,the average recovery was 78.63％～94.17％,and the RSD was 2.60％～4.43％ ; ③when the level of paddy and brown rice were 0.035 2～3.52mg/kg,the average recovery were 72.01％～97.42％,81.78％～97.06％,respectively,and the RSDs were 3.52％～5.91％,1.69％～10.1％,respectively.The results showed that when the dosage of the pesticide was 0.352～35.2μg/mL,the limit of detection （LOD） was 4.2x10-10g,and the minimum detectable concentration was 0.008 8mg/kg.The method is simple,rapid,and accurate quantitative determination.It can be effectively applied on tricyclazole residues in rice.