甘蓝结球过程中内源激素含量与裂球性的关系

以4份耐裂球性不同的甘蓝高代自交系为试验材料，采用间接酶联免疫（ELISA）检测技术，对甘蓝结球过程中球叶中的生长素（IAA）、赤霉素（GA₃）、玉米素核苷（ZR）和脱落酸（ABA）4大类内源激素的含量和比值变化进行了分析，以探讨甘蓝裂球性与内源激素含量的关系。结果表明：在整个结球期，耐裂球材料各个时期的IAA、GA₃含量均高于易裂球材料。ZR含量对甘蓝的裂球性影响不大。ABA含量在4种内源激素中含量值最大，而且耐裂球材料与易裂球材料间的动态差异较大。叶球生长后期（IAA+GA₃+ZR）/ABA比值高可使叶球保持较高的生长势头，从而承受内部生长应力的能力较大，减少叶球开裂。

Relationship between Endogenous Hormone Contents and Head-splitting Character during Brassica oleraca L.var.capitata L.Vegetative Period

This study was designed to elucidate the relationship between endogenous hormone contents and head-splitting character of Brassica oleraca L.var.capitata L.Enzyme-linked immunosorbent assays(ELISA)was used to determine the contents of indoleacetic acid(IAA),gibberellin(GA₃),cytokinin(ZR)and abscisic acid(ABA)and to analyze their ratio changes,so as to probe into the relation between head-splitting and endogenous hormone.These endogenous hormones were taken from head leaves of 4 Brassica oleraca L.var.capitata L.cultivars which differ in splitting susceptibility during vegetative period.The results showed that during the whole vegetative period,the contents of IAA and GA₃ in splitting-resistant cultivars were higher than those of splitting-susceptible cultivars.ZR content had less effect on head-splitting of Brassica oleraca L.var.capitata L.ABA content was the highest among the 4 endogenous hormones,and there were great dynamic differences between splitting-resistant and splitting-susceptible cultivars.The high ratio of(IAA+GA₃+ZR)to ABA at the late stage could keep the Brassica oleraca L.var.capitata L.head in a strong growing vigor,thus reduce the head splitting probability.