Determination of protein concentrations and their molecular weight in tears from cats with normal corneas and cats with corneal sequestrum. |
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| Authors: | H J Davidson J A Gerlach R W Bull |
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| Institution: | Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201. |
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| Abstract: | Protein concentration was determined, using the Bradford technique, in tears from cats with normal corneas and from cats with corneal sequestrum. Tears from the former group contained 5.81 +/- 2.29 mg of protein/ml; those from corneal sequestrum-affected cats contained 6.21 +/- 2.21 mg/ml. Difference between the 2 values was not significant. Molecular weight determination was made, using 4 to 20% sodium dodecyl sulfate-polyacrylamide gels. Molecular mass of proteins ranged from 263 to 14 kDa. There was no detectable difference in the band patterns for the 2 groups. |
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