| 长期冻存对昆虫细胞系SL2和NIH-SaPe-4活性的影响 |
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| 引用本文： | 丁伟峰,马艳,冯颖,张欣,马涛.长期冻存对昆虫细胞系SL2和NIH-SaPe-4活性的影响[J].林业科学研究,2010,23(5):666-670. |
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| 作者姓名： | 丁伟峰 马艳 冯颖 张欣 马涛 |
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| 作者单位： | 中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南,昆明,650224 |
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| 基金项目： | 林业公益性行业科研专项(4-38)、国家林业局948项目(2002-52) |
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| 摘 要： | 对2种双翅目昆虫细胞系(黑腹果蝇胚细胞系SL2以及麻蝇胚细胞系NIH-SaPe-4)38个月长期液氮深低温保存效果进行了研究,通过对冻存时间和保护剂对2种细胞系冻后活力、圆度以及恢复时间影响的测定,比较了3种不同配方冻存保护液的效果。结果表明:2种昆虫细胞系冻后活性均随着冻存时间的增长而逐渐降低。长期冻存期中,使用90%胎牛血清(FBS)+10%二甲基亚砜(DMSO)冻存SL2效果较好,NIH-SaPe-4使用10%DMSO即可满足冻存的需要,而无需额外添加高浓度FBS。甘油不适用于这2种昆虫细胞系的长期保存。
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| 关 键 词： | 冷冻保护剂 冻存时间 昆虫细胞 细胞活力 细胞圆度 |
| 收稿时间： | 2010/5/10 0:00:00 |
Effects of Long-term Cryopreservation on the Cytoactive of Two Insect Cell Lines SL2 and NIH-SaPe-4 |
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| DING Wei-feng,MA Yan,FENG Ying,ZHANG Xin and MA Tao.Effects of Long-term Cryopreservation on the Cytoactive of Two Insect Cell Lines SL2 and NIH-SaPe-4[J].Forest Research,2010,23(5):666-670. |
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| Authors: | DING Wei-feng MA Yan FENG Ying ZHANG Xin and MA Tao |
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| Institution: | Research Institute of Resource Insects, Chinese Academy of Forestry,Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration,Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry,Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration,Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry,Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration,Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry,Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration,Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry,Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration,Kunming 650224, Yunnan, China |
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| Abstract: | In order to evaluate the effects of different cryoprotectants and durations on post-thaw cytoactives (including cell viability, circularity and recovery time) of two dipteral insect cell lines, 3 kinds of cryoprotectants (Formula I: 10% DMSO + 90% FBS; Formula II: 10% DMSO; Formula III: 10% glycerol) were used to preserve the cells of SL2 from embryo of Drosophila melanogaster and NIH-SaPe-4 from embryo of Sarcophaga peregrine in liquid nitrogen separately. The results showed that the post-thaw viability of these two cell lines decreased with the cryopreservation time expended. In long-term cryopreservation, the effect of Formula I for preserving SL2 cells was better than the others. Formula II was suitable for NIH-SaPe-4. Using glycerol as cryoprotectant was unsuitable for these two insect cell lines. |
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| Keywords: | cryoprotectant cryopreserving duration insect cells cell viability cell circularity |
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