| 马铃薯试管薯工厂化生产优化试验 |
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| 引用本文： | 吴京姬,金顺福,姜成模,玄春吉,李玉子,康哲秀,郎贤波,南哲佑. 马铃薯试管薯工厂化生产优化试验[J]. 中国马铃薯, 2011, 25(4) |
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| 作者姓名： | 吴京姬 金顺福 姜成模 玄春吉 李玉子 康哲秀 郎贤波 南哲佑 |
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| 作者单位： | 延边农业科学院,吉林龙井,133400 |
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| 基金项目： | 国家农业部马铃薯产业技术体系资助(CARS-10-ES07) |
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| 摘 要： | 为使马铃薯试管薯工厂化生产,对试管薯生产参试进行了优化。试验结果表明:在2MS培养基中壮苗,是获得试管薯高产的必要前提。在诱导培养基MS+6BA0.5 mg/L+8%白糖液体培养基上黑暗处理2周后放入自然光下培养可提高试管薯的产量。当容器使用220 mL培养瓶时,诱导培养液量为50 mL为宜,并且每瓶放入20个茎段效果最好。试管苗去掉顶部后进行试管薯生产可提高成苗率和试管薯产量。
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| 关 键 词： | 马铃薯 试管薯诱导 光照处理 培养液 节位 |
Optimization for Microtuber Bunch Production |
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| WU Jingji,JIN Shunfu,JIANG Chengmo,XUAN Chunji,LI Yuzi,KANG Zhexiu,LANG Xianbo,NAN Zheyou. Optimization for Microtuber Bunch Production[J]. Chinese Potato, 2011, 25(4) |
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| Authors: | WU Jingji JIN Shunfu JIANG Chengmo XUAN Chunji LI Yuzi KANG Zhexiu LANG Xianbo NAN Zheyou |
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| Affiliation: | WU Jingji,JIN Shunfu,JIANG Chengmo,XUAN Chunji,LI Yuzi,KANG Zhexiu,LANG Xianbo,NAN Zheyou(Yanbian Academy of Agricultural Sciences,Longjing,Jilin 133400,China) |
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| Abstract: | In order to carry on the bunch production of microtubers,the optimization was made for the production parameters.Using 2 times of MS medium was a necessary premise for achieving robust plantlets in vitro and thereby a high yield of microtubers.Yield of microtubers was increased when plantlets in vitro were cultured in the inducing liquid medium MS + 6 BA 0.5 mg / L + 8% white sugar in the dark for two weeks and then under natural light.The best effects were achieved when 50 mL of inducing medium were added ... |
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| Keywords: | potato microtuber induction light treatment liquid medium segment position |
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