| 马铃薯PLRV的RT-LAMP检测方法的构建 |
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| 引用本文： | 乔楠,曹佳. 马铃薯PLRV的RT-LAMP检测方法的构建[J]. 中国马铃薯, 2011, 25(5) |
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| 作者姓名： | 乔楠 曹佳 |
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| 作者单位： | 1. 榆林学院生命科学学院,陕西榆林,719000
2. 北京真思维生物科技有限公司,北京,100044 |
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| 摘 要： | 为了建立高效、便捷且成本较低的检测方法以应用于马铃薯卷叶病毒(Potato leaf roll virus,PLRV)检疫防控工作,本研究构建了PLRV的逆转录环介导恒温扩增反应(Reverse transcription loop-mediated isothermal amplification,RT-LAMP)检测方法。结果表明:本研究构建的马铃薯PLRV的RT-LAMP检测方法,最低检测下限为10 copies/mL,与PVX、PVM、PVV、PVS、CMV等其他马铃薯病毒无交叉反应,具有良好的特异性,为马铃薯病毒检测技术应用奠定一定基础。
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| 关 键 词： | 马铃薯 马铃薯卷叶病毒 逆转录环介导恒温扩增反应 |
Development of a Rapid Detection Method for Potato Leaf Roll Virus by a Reverse Transcription Loop-Mediated Isothermal Amplification Assay |
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| QIAO Nan,CAO Jia. Development of a Rapid Detection Method for Potato Leaf Roll Virus by a Reverse Transcription Loop-Mediated Isothermal Amplification Assay[J]. Chinese Potato, 2011, 25(5) |
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| Authors: | QIAO Nan CAO Jia |
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| Affiliation: | QIAO Nan1,CAO Jia2*(1.College of Life Science,Yulin University,Yulin,Shaanxi 719000,China,2.Beijing Genesway Bio-tech Co.Ltd,Beijing 100044,China) |
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| Abstract: | In order to develop high-effective,convenient and low cost method for the detection of potato leaf roll virus(PLRV) for inspection and control of this disease,a PLRV reverse transcription loop-mediated isothermal amplification(RT-LAMP) assay was carried out for detecting PLRV.The results showed that RT-LAMP method was effective,and the lowest detection limit was 10 copies / mL.No cross-reactivity was observed with other viruses(PVX,PVM,PVV,PVS,and CMV).Therefore,it had good specificity and laid a good found... |
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| Keywords: | potato potato leaf roll virus reverse transcription loop-mediated isothermal amplification |
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