| 猪TOLL样受体2基因在CHO-K1细胞中的表达 |
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| 引用本文： | 房永祥,景志忠,陈国华,段凤云,蒙学莲,窦永喜.猪TOLL样受体2基因在CHO-K1细胞中的表达[J].甘肃农业大学学报,2008,43(1):14-17. |
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| 作者姓名： | 房永祥 景志忠 陈国华 段凤云 蒙学莲 窦永喜 |
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| 作者单位： | 1. 甘肃农业大学,甘肃,兰州,730070
2. 中国农业科学院兰州兽医研究所,甘肃省动物寄生虫病重点实验室,家畜疫病病原生物学国家重点实验室,甘肃,兰州,730046 |
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| 基金项目： | 国家高技术研究发展计划“863”计划——“新型高效农业生物药物载体和新制剂创制”(2006AA10A203). |
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| 摘 要： | 将含猪TLR2(猪Toll样受体2,pTLR2)基因的真核表达载体PcDNA3.1/CT-GFP-pTLR2,采用脂质体法转染到CHO-K1细胞,用G418持续筛选获得了阳性细胞克隆.阳性细胞克隆系经PCR和RT-PCR检测表明:pTLR2基因得到稳定整合并能成功地转录.荧光显微镜观察,转染细胞可见绿色荧光,表明获得了表达pTLR2的克隆细胞系.
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| 关 键 词： | 猪Toll样受体2 CHO-K1细胞 转染 |
| 文章编号： | 1003-4315（2008）01-0014-04 |
| 收稿时间： | 2007-04-16 |
| 修稿时间： | 2007年4月16日 |
Expression of porcine Toll-like receptor TLR2 gene in the CHO-K1 cells |
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| FANG Yong-xiang,JING Zhi-zhong,CHEN Guo-hua,DUAN Feng-yun,MENG Xue-lian,DOU Yong-xi.Expression of porcine Toll-like receptor TLR2 gene in the CHO-K1 cells[J].Journal of Gansu Agricultural University,2008,43(1):14-17. |
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| Authors: | FANG Yong-xiang JING Zhi-zhong CHEN Guo-hua DUAN Feng-yun MENG Xue-lian DOU Yong-xi |
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| Abstract: | The recombinant plasmid PcDNA3.1/CT-GFP-pTLR2 was transfected into the CHO-K1 cells by lipofectin and the positive cell clone was obtained after screening by G418 for 2~3 weeks.Analysis of the exogenous gene by PCR and RT-PCR indicated that the pTLR2 gene was integrated into the CHO-K1 cells.The expression of pTLR2-GFP fusion protein was observed by intensity fluorescence microscope at different time after transfection.The results indicated that pTLR2-GFP fusion protein was successfully expressed in the cells and these cells could be further used to investigate the functions of pTLR2. |
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| Keywords: | porcine Toll-like receptor 2 CHO-K1 cells transfection |
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