The induction of 1,3-β-glucanases and other enzymes in groundnut leaves infected with Cercospora arachidicola |
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Authors: | S. Roulin A. J. Buchala |
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Abstract: | Infection of groundnut leaves with the early leaf spot pathogen Cercospora arachidicola leads to a marked increase in extracellular 1,3-β-glucanase activity, limited to the infected tissue. Three isoforms of low molecular weight and extreme pI values, typical of pathogenesis-related proteins, were induced. These β-glucanases, when acting together, were capable of degrading the pathogen cell wall in vitro. Glucanases from homogenates of infected leaf tissue were partially purified by ion-exchange chromatography to give enzymes with molecular weights of 35, 32 and 20 kDa and pI values of 3·8, 3·6 and > 9, respectively. They were electrophoretically identical to the β-glucanases found in the intercellular washing fluid. Treatment of groundnut plants with 200 μM mercuric chloride induced the accumulation of identical extracellular β-glucanases. During the course of the infection an increase in peroxidase activity was also observed, but chitinase activity remained more or less constant. |
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Keywords: | DEAE, diethylaminoethyl dpi, days post-infection Hepes, 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid ICWF, intercellular washing fluid PR, pathogenesis-related Tris, tris-(hydroxymethyl)-aminomethane SDS-PAGE, sodium dodecylsulphate-polyacrylamide gel electrophoresis |
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