| 橡胶树炭疽病菌Cap20基因的克隆和序列分析 |
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| 引用本文： | 林春花,李超萍,李博勋,周维,黄贵修.橡胶树炭疽病菌Cap20基因的克隆和序列分析[J].热带作物学报,2012,33(11):1991-1995. |
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| 作者姓名： | 林春花 李超萍 李博勋 周维 黄贵修 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带农业有害生物监测与控制重点实验室 ,海南海口,571101 |
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| 基金项目： | 国家天然橡胶产业技术体系资金资助项目（No. CARS-34-GW8）；国家自然科学基金项目（No. 31201468）。 |
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| 摘 要： | 根据鳄梨胶孢炭疽菌Cap20基因序列设计引物,利用同源基因克隆法获得橡胶树胶孢炭疽菌和尖孢炭疽菌Cap20基因,同时进行核苷酸序列、氨基酸序列及聚类分析。结果表明:2种橡胶树炭疽菌的Cap20基因均含2个外显子和1个内含子,编码183个氨基酸;橡胶树胶孢炭疽菌与鳄梨胶孢炭疽菌同源性最高,其核苷酸序列同源性达90.3%,氨基酸序列同源性达98.9%;来自橡胶树的2种炭疽菌Cap20基因序列存在较大差异,其核苷酸水平同源性仅为73.8%,氨基酸水平同源性为79.8%;炭疽菌CAP20和绿僵菌MPL1蛋白结构具有42%的同源性,所含蛋白结构域相似,推测炭疽菌Cap20基因可能与绿僵菌Mpl1基因功能具有相似性。
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| 关 键 词： | 橡胶树 尖孢炭疽菌 胶孢炭疽菌 Cap20 基因克隆 |
Cloning and Sequences Analysis of Gene Cap20 From the Pathogens Colletotrichum spp.Causing Leaf Disease of Rubber Tree |
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| LIN Chunhu,LI Chaoping,LI Boxun,ZHOU Wei and HUANG Guixiu.Cloning and Sequences Analysis of Gene Cap20 From the Pathogens Colletotrichum spp.Causing Leaf Disease of Rubber Tree[J].Chinese Journal of Tropical Crops,2012,33(11):1991-1995. |
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| Authors: | LIN Chunhu LI Chaoping LI Boxun ZHOU Wei and HUANG Guixiu |
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| Institution: | Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture / Hainan Key Laboratory for Monitoring and Control of Tropical Agricu;Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture / Hainan Key Laboratory for Monitoring and Control of Tropical Agricu;Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture / Hainan Key Laboratory for Monitoring and Control of Tropical Agricu;Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture / Hainan Key Laboratory for Monitoring and Control of Tropical Agricu;Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture / Hainan Key Laboratory for Monitoring and Control of Tropical Agricu |
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| Abstract: | |
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| Keywords: | Rubber tree Colletotrichum acutatum Collectotrichum gloeosporioides Cap20 Gene cloning |
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