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康乃馨ACC氧化酶反义基因遗传转化康乃馨的研究
引用本文:张树珍,汤火龙,杨本鹏,刘飞虎. 康乃馨ACC氧化酶反义基因遗传转化康乃馨的研究[J]. 园艺学报, 2003, 30(6): 699-702
作者姓名:张树珍  汤火龙  杨本鹏  刘飞虎
作者单位:( 中国热带农业科学院热带作物生物技术国家重点实验室,海口571101; 云南大学生命科学学院,昆明650091)
基金项目:中国博士后科学基金项目(2002C0003M);云南省自然科学基金项目(2002C0002M)
摘    要: 在建立康乃馨从愈伤组织到完整植株的高频再生体系的基础上,用花特异表达启动子驱动的康乃馨ACC氧化酶反义基因通过农杆菌介导法对康乃馨进行遗传转化,获得了3株转基因植株.经多重PCR及PCR-Southem杂交检测,证实康乃馨ACC氧化酶的反义基因已整合进康乃馨的基因组中。

关 键 词:康乃馨  ACC氧化酶  花特异表达启动子  反义基因  遗传转化
文章编号:0513-353X(2003)06-0699-04
收稿时间:2003-04-17
修稿时间:2003-04-17

Genetic Transformation of Carnation with Its ACC Oxidase Antisense Gene
Zhang Shuzhen,Tang Huolong,Yang Benpeng,and Liu Feihu. Genetic Transformation of Carnation with Its ACC Oxidase Antisense Gene[J]. Acta Horticulturae Sinica, 2003, 30(6): 699-702
Authors:Zhang Shuzhen  Tang Huolong  Yang Benpeng  and Liu Feihu
Abstract:The high frequency regeneration system of carnation from leaf to callus to plandets was established. Then genetic transformation of the carnation with the ACO antisense gene, which controlled by flower-specific expression promoter (PchsA) was performed via agrobacterium mediated method and obtained 3 of transgenic plants via the pathway of regeneration plandets from callus at last. The results of the PCR and PCR-Southern hybridization analysis showed that the carnation ACC oxidase antisense gene was integrated into carnation genomes. It will be a good foundation for improvement the longevity of carnation flowers.
Keywords:Carnation  ACC oxidase  Antisense gene  Genetic transformation
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