Requirement for the SLP-76 adaptor GADS in T cell development |
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Authors: | Yoder J Pham C Iizuka Y M Kanagawa O Liu S K McGlade J Cheng A M |
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Affiliation: | Medical Scientist Training Program, Washington University School of Medicine, St. Louis, MO 63110, USA. |
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Abstract: | GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS- CD4-CD8- thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS- thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS- thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling. |
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