高粱DNA的提取纯化及抗丝黑穗病基因的初步分析

[目的]为快速选育抗病高粱新品种提供理论依据。[方法]采用CTAB法提取高粱基因组DNA,运用RAPD分子标记技术对高粱的抗丝黑穗病基因进行初步分析。[结果]琼脂糖凝胶电泳检测结果表明,采用CTAB法提取高粱叶片DNA有较好的提取效果。通过对70个随机引物进行筛选,获得了34个具有多态性扩增谱带的RAPD引物,确定为适宜引物。34个适宜引物共扩增出107条谱带,平均每个引物扩增出3.1条谱带,使高粱基因组呈现出1种多态性。[结论]高粱抗丝黑穗病基因的RAPD扩增谱带集中分布在600～3 000 bp。

Extraction and Purification of Sorghum DNA and Preliminary Analysis of Head Smut Resistance gene on in Sorghum by RAPD Marker

[Objective] The research aimed to provide theoretical basis for breeding new sorghum varieties with disease resistance rapidly.[Method] Genomic DNA was extracted from sorghum by using CATB method and head smut resistance gene in sorghum was preliminarily analyzed by using RAPD molecular marker technology.[Result] The results of detection by agarose gel electrophoresis indicated that extracting DNA from the leaves of sorghum by CATB method had better extraction effects.Thirty-four RAPD primers with polymorphic amplified bands were obtained through screening 70 random primers,which were confirmed as suitable primers.One hundred and seven bands were amplified by 34 suitable primers.Each primer amplified 3.1 bands averagely,which made the genome of sorghum show a kind of polymorphism.[Conclusion] RAPD amplified bands of head smut resistance gene in sorghum were concentrated in 600~3 000 bp.