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The optimal time to stain for noncytopathic bovine virus diarrhea field sample virus using indirect fluorescent antibody technique |
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| Authors: | L.F Ayanwale J Fahrmann D.W Johnson R.K Anderson J.M.B Kaneene |
| Affiliation: | * Division of Epidemiology, School of Public Health, 1360 Mayo Building, University of Minnesota, Minneapolis, MN 55455, U.S.A. ‡ Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, U.S.A. |
| Abstract: | The use of an indirect fluorescent antibody (IFA) technique has been further explored to determine the optimum day(s) to stain for a noncytopathic bovine virus diarrhea virus from pooled and individual field samples. The IFA test further confirmed that under the experimental conditions of this study, somewhere between days 4 and 5 would be an optimum time to stain for a noncytopathic BVD virus which was present in tissues of suspected animals and diluted with minimal essential media. |
| Keywords: | Indirect fluorescent antibody technique noncytopathic strains bovine virus diarrhea minimum essential mediaAuthor Keywords: Technique d'immunofluorescence indirecte souches non cytopathogènes virus de la maladie des muqueuses milieux minima essentiels |
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