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利用多因子正交试验建立桑的无菌快繁体系
引用本文:李想韵,朱鸿,饶灿,李鹏,宋锋. 利用多因子正交试验建立桑的无菌快繁体系[J]. 北方园艺, 2009, 0(10)
作者姓名:李想韵  朱鸿  饶灿  李鹏  宋锋
作者单位:李想韵,朱鸿,饶灿,李鹏,宋锋,彭江,雷桅(西南大学,生命科学学院,四川,重庆,400715);孙敏(西南大学生命科学学院及三峡库区生态环境教育部重点实验室,400715) 
基金项目:三峡库区生态环境教育部重点实验室开放基金资助项目 
摘    要:以桑侧枝茎段为材料,利用L9(34)正交设计探讨培养基种类和激素配比对桑茎段丛生芽诱导效率的影响,并对影响桑外植体生根的主要因子进行了研究.结果表明:A2B2C1为诱导出芽的最佳培养基与激素组合,并且可同时实现芽增殖;生根培养基为WPM+1.0 mg/L IBA.

关 键 词:  正交没计  不定芽诱导  生根

Application of Orthogonal Design in Establishing Sterile Rapid Propagation System of Mulberry
LI Xiang-yun,ZHU Hong,RAO Can,LI Peng,SONG Feng,PENG Jiang,LEI Wei,SUN Min. Application of Orthogonal Design in Establishing Sterile Rapid Propagation System of Mulberry[J]. Northern Horticulture, 2009, 0(10)
Authors:LI Xiang-yun  ZHU Hong  RAO Can  LI Peng  SONG Feng  PENG Jiang  LEI Wei  SUN Min
Affiliation:LI Xiang-yun1,ZHU Hong1,RAO Can1,LI Peng1,SONG Feng1,PENG Jiang1,LEI Wei1,SUN Min1,2(1.College of Life Science,Southwest University,Chongqing 400715,China,2.Key Laboratory of Eco-environments in Three Gorges Reservoir Region Ministry of Education,China)
Abstract:The lateral branches of Mulberry were taken as explants,effects of various medium and different plant hormones on caespitose buds induction was studied by L9(34) orthogonal design,and the roots induction was researched.The results showed that: the optimal medium for buds induction was A2B2C1,meanwhile the breed up of casespitose buds was realized;the cultural medium for growth of roots was WPM+1.0 mg/L IBA.
Keywords:Muberry  Orthogonal design  Casespitose buds induction  Roots growth  
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