茶毛虫核型多角体病毒对茶毛虫的致病性研究

研究了茶毛虫核型多角体病毒（Euproctis pseudoconspersa nuclear polyhedrosis virus,简称EpNPV）的室内外增殖、分离粗提,以及对茶毛虫（Euproctis pseudoconspersa Strand）室内毒力测定、田间防治效果和病毒电镜检测。采用室内离体茶树嫩枝水培,106βPIB/ml EpNPV喷雾接种2~3龄茶毛虫,或大田直接喷雾处理2~3龄茶毛虫,在罹病幼虫液化前及时收集虫尸,完成病毒增殖。106βPIB/ml、107βPIB/ml、108βPIB/ml EpNPV室内处理第12天致病率分别为43.60％、76.13％和64.68％;第14天致病率分别为59.17％、95.71％和95.70％。大田试验107βPIB/ml喷雾处理第15天防治效果达78.11%。经透射电镜观察田间防治大量感病茶毛虫虫尸,证实为EpNPV致病死亡。

The Pathogenicity of Euproctis Pseudoconspersa Nuclear Polyhedrosis Virus on the Larvae of Tea Tussork (Euproctis pseudoconspersa Strand)

The reproduction, extraction, pathogenicity to Tea Tussork Moth and electron micrograph testing of Euproctis pseudoconspersa Nuclear Polyhedrosis Virus(EpNPV) was investigated. The EpNPV was propagated through Inoculating 2~3 instar larvae of Euproctis pseudoconspersa, by raged with EpNPV at the concentration 106 PIB/ml, or inoculating 2~3 instar larvae in field condition. Inoculation the larvae with 106 PIB/ml, 107 PIB/ml, and 108 PIB/ml of EpNPV, the corrected mortalities were 43.60%, 76.13% and 64.68% on day 12 respectively, and reached 59.17%, 95.71%, and 95.70% on day 14 respectively. The mortality was 78.11% on day 15 treated with EpNPV(107 PIB/ml) under field condition. Virus of the EpNPV from the field dead larvae could be watched through the electron microscope.