| 春兰AGL6基因的克隆及实时定量表达分析 |
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| 引用本文： | 孙崇波,向林,施季森,胡凤荣,郭方其,秦德辉. 春兰AGL6基因的克隆及实时定量表达分析[J]. 分子植物育种, 2010, 8(5). DOI: 10.3969/mpb.008.000939 |
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| 作者姓名： | 孙崇波 向林 施季森 胡凤荣 郭方其 秦德辉 |
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| 作者单位： | 浙江省农业科学院园艺所,杭州,310021;南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京,210037;浙江省农业科学院园艺所,杭州,310021;南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京,210037 |
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| 基金项目： | 浙江省自然科学基金项目,浙江省科技计划项目 |
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| 摘 要： | 本研究采用RT-PCR结合RACE技术从春兰(Cymbidium goeringii)中分离到一个AGL6基因。序列分析表明,该基因含有一个729bp长的开放阅读框(ORF),共编码242个氨基酸。系统进化树分析显示,该基因属于MADS-box基因家族AP1/AGL9组的AGL6同源基因,其编码的蛋白与其它植物AGL6类蛋白具有较高的同源性,命名为CgAGL6(基因登录号为HM208533)。实时荧光定量表达分析表明,CgAGL6基因春兰不同组织中均有表达,其中在唇瓣、花芽和子房中的表达量较高,在花瓣和萼片中的表达量次之,在根、叶和蕊柱中的表达量最低,显示了CgAGL6基因可能在春兰成花转变和花器官的形成过程中起着重要作用。
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| 关 键 词： | 春兰 AGL6基因 实时荧光定量表达 花发育 |
Cloning and Real-time Expression Analysis of AGL6 Gene from Cymbidium goeringii |
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| Sun Chongbo,Xiang Lin,Shi Jisen,Hu Fengrong,Guo Fangqi,Qin Dehui. Cloning and Real-time Expression Analysis of AGL6 Gene from Cymbidium goeringii[J]. Molecular Plant Breeding, 2010, 8(5). DOI: 10.3969/mpb.008.000939 |
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| Authors: | Sun Chongbo Xiang Lin Shi Jisen Hu Fengrong Guo Fangqi Qin Dehui |
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| Affiliation: | Sun Chongbo 1 Xiang Lin1 Shi Jisen2 HuFengrong 2 Guo Fangqi 1 QinDehui 1 1 Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou,310021,2 Key Laboratory of Forest Genetics & Biotechnology,Nanjing Forestry University,Ministry of Education,Nanjing,210037 |
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| Abstract: | In this research,a novel AGL6 gene was isolated from Cymbidium goeringii by RT-PCR and RACE-PCR techniques.Sequence analysis showed the gene contained an open reading frame of 729 bp encoding a putative protein of 242 amino acids.Homology analysis showed that the deduced protein was high identity with other AGL6 proteins from different species,named CgAGL6(GenBank accession No.HM208533).Phylogenetic tree analysis also indicated that CgAGL6 belongs to AGL6 clade of AP1/AGL9 subfamily.Real-time quantitative P... |
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| Keywords: | Cymbidium goeringii AGL6 gene Real-time quantitative PCR Floral development |
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