超声波辅助提取多穗石柯根皮苷研究

旨在优选超声波辅助提取多穗石柯根皮苷工艺。采用控制单一变量法，分别确定超声波辅助提取多穗石柯根皮苷的料液比、乙醇浓度、浸提温度、浸提时间较优范围，然后采用L₉(3⁴)正交实验及最优组合验证实验方案优化筛选最佳提取工艺。影响多穗石柯根皮苷提取得率的因素影响大小依次为：提取时间>乙醇浓度>提取温度>料液比，最佳提取工艺条件为提取温度45℃、料液比1:30、70%乙醇浓度、提取时间35 min，在此提取工艺条件下，多穗石柯根皮苷提取得率1.421%，提取物中根皮苷纯度为3.797%，验证性实验的相对偏差RSD=0.881%，说明该最佳提取工艺重复性和稳定性较好。

Study on Ultrasonic Assisted Extraction Technology of Phloridzin in Lithocarpus polysachyus Rehd

The aim was to prioritize the ultrasonic-assisted extraction technology of phloridzin in Lithocarpus polysachyus Rehd.. By controlling the change of mono factors, the optimal range of the solid-liquid ratio, ethanol concentration, extraction temperature and extraction time were validated with ultrasonic-assisted extraction in Lithocarpus polysachyus Rehd.. And then L₉(3⁴) orthogonal design and verification experiments were used to determine the optimal extraction process. Factors influencing the extraction yield of phloridzin in Lithocarpus polystachyus were as follows: extraction time > ethanol concentration> extraction temperature > solid-liquid ratio. The optimal extraction conditions were as follows, extraction temperature 45℃, solid-liquid ratio 1:30, extraction solvent 70% ethanol, and extraction time 35 min. Under these extraction process conditions, the extraction yield of phloridzin was 1.421% and the content of phloridzin was 3.797%. The RSD of verified experiment was 0.881%, which indicated that the optimal extraction process had good reproducibility and stability.