叶用莴苣2个Hsp70基因与其抽薹的相关性分析

为探究叶用莴苣2个Hsp70基因LsHsp70-2711和LsHsp70-3711与叶用莴苣的抽薹是否相关，采用实时荧光定量PCR技术(RT-PCR)，以2个品种的叶片为试材，分析这2个基因在耐热型品种G-S59和热敏型品种P-S11 2个叶用莴苣品种抽薹前后的相对表达量。研究结果表明：首先，这2个Hsp70基因在抽薹前后的表达量表现出显著差异，所以推测与叶用莴苣的抽薹性具有一定关联；其次，LsHsp70-2711基因在2个叶用莴苣品种中的表达趋势均为抽薹前显著高于抽薹后，且在相同处理下G-S59中的表达量又明显高于P-S11中的表达量，LsHsp70-3711基因与LsHsp70-2711基因在2个品种中的表达情况相反。叶用莴苣热激蛋白基因LsHsp70-2711和LsHsp70-3711除与叶用莴苣的耐热性相关外，还与其抽薹性具有一定相关性。

Correlation Analysis of Bolting and Two Hsp70 Genes in Lactuca sativa L.

Based on a heat-resistant type G-S59 and a heat-sensitive type P-S11, in this study, we analyzed the relationship between bolting and two heat shock protein genes in Lactuca sativa L.(LsHsp70-2711 gene and LsHsp70-3711 gene) with the help of real-time quantitative PCR (qRT-PCR). The result showed that the expression of the two Hsp70 genes in lettuce varied significantly before and after bolting, based on which we could conjecture that there was a relationship between bolting and the two Hsp70 genes in lettuce. In addition, in either type, the relative expression of LsHsp70-2711 after bolting was evidently higher than that beforehand. Moreover, when under the same treatment, the expression of LsHsp70-2711 gene in heat-resistant type G-S59 was much higher than that in heat-sensitive type P-S11 while the expression of LsHsp70-3711 gene had the opposite result. Therefore we deduced that the two Hsp70 genes not only had correlation with heat-resistance in lettuce, but also had some extent relationships with bolting.