不同方法提取的鹧鸪茶多酚抗氧化及抑菌性比较

为筛选适合鹧鸪茶多酚的提取方法,在热水浸提法、有机溶剂浸提法和纤维素酶辅助浸提法提取鹧鸪茶多酚基础上,采用DPPH自由基法、ABTS自由基法、羟自由基法、超氧阴离子法和还原力法评价多酚提取物的抗氧化性;滤纸片扩散法和二倍稀释法测定多酚提取物的抑菌性.结果表明,热水浸提法、有机溶剂浸提法和纤维素酶辅助浸提法提取鹧鸪茶多酚...

Antioxidant and Bacteriostasis Activity of Flavanoid from Mallotus oblongifolius by Different Extraction Methods

The aim was to select the suitable method for extracting the polyphenols from Mallotus oblongifolius.The polyphenols were extracted by hot water extraction,organic solvent extraction and cellulase-assisted extraction.The antioxidant activity of the extract density was evaluated by scavenging DPPH,ABTS,hydroxyl,superoxide anion radical and reducing force methods.The antibacterial activity of the polyphenol extracts was determined by filter paper diffusion method and double dilution methods.The yield of polyphenols by the hot water extraction method,organic solvent extraction method and cellulase-assisted extraction method was(10.31±0.42)%,(9.79±0.38)%,and(10.88±0.52)%,respectively,and the content was(44.72±2.26)%,(39.54±1.78)%,and(47.07±2.52)%.The polyphenol extracts extracted by the three methods had strong antioxidant properties.The cellulose-assisted extraction of the polyphenols from M.oblongifolius(CFC)had the strongest ability to scavenge DPPH radical,and its half inhibitory concentration(IC₅₀)was(0.0034±0.0002)mg/mL.The hot water-extracted polyphenols from M.oblongifolius(WFC)had the strongest ability to scavenge ABTS radical,hydroxyl radical,and superoxide anion radical,and the IC₅₀ values was(0.066±0.004),(0.069±0.004),(0.127±0.009)mg/mL.The polyphenol extracts extracted by the three methods had a good inhibitory effect on four bacteria.Among them,WFC had a good inhibitory effect on Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Pseudomonas aerugino,the inhibitory zone diameter was(12.34±1.01),(12.16±0.95),(2.12±0.15),(6.12±0.36)mm,and the minimum inhibitory concentration(MIC)was 3.13,3.13,12.50,6.25 mg/mL.Comprehensive consideration,the hot water extraction method is more suitable for the extraction of polyphenols from M.oblongifolius.