沉香叶黄酮稳定性及对H2O2诱导HepG2细胞氧化损伤的保护作用

研究沉香叶黄酮稳定性及对H2O2诱导HepG2细胞氧化损伤的保护作用.通过测定溶液吸光值,探讨温度、光照、金属离子、pH值对沉香叶黄酮稳定性的影响.建立H2O2诱导HepG2细胞氧化损伤模型,将细胞分为正常组、模型组以及沉香叶黄酮低、中、高剂量组,采用水溶性四唑盐法检测细胞增殖率,2′,7′-二氢二氯荧光黄双乙酸钠法(...

Stability and Protection Against H2O2 Induced Oxidative Stress Injury in HepG2 of Flavonoids from Aloes Leaves

The effects of temperature, light, metal ions, and pH on the stability of aloes leaves (FAL) were explored to evaluate the stability of the flavonoids from FAL and the protective effect against oxidative damage induced by H₂O₂ in HepG2 cells. A model of oxidative damage to HepG2 cells induced by H₂O₂ was established. Cells were divided into normal group, oxidative stress model group, and low-, medium- and high-dose FAL treatment groups. The cell proliferation rate was measured by the water soluble tetrazolium method (WST-1) assay. The intracellular reactive oxygen specisis (ROS) was determined by the dichloro - dihydro - fluorescein diacetate (DCFH-DA) assay. The content of lactate dehydrogenase (LDH), the level of malondiadehycle (MDA) and the activity of superoxidedimutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were detected by biochemical kits. The results showed that FAL were more stable under temperature 70 ℃, pH 3-7, in the Na⁺, K⁺, Mg²⁺, Ca²⁺ metal ions, in the dark environment and energy-saving lighting. FAL significantly increased the cell viability and activity of SOD, CAT and GSH-Px in cells. In addition, FAL also effectively decreased the intracellular levels of ROS and MDA, reduced the spread of LDH out of the cell. FAL had certain stability under low temperature, weak acid, some metal ions, and low light conditions. Consequently, FAL could protect HepG2 cells against H₂O₂-induced oxidative damage, and its underlying mechanism maight be related to regulating the cellular redox system, scavenging intracellular ROS, and increasing the activities of intracellular antioxidant enzymes.