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| 金鱼草基因转化中卡那霉素筛选压力的确定 | |
| 引用本文： | 黄俊轩,倪志明,桂毓,解瑞彬,刘艳军,杨静慧,吕成功. 金鱼草基因转化中卡那霉素筛选压力的确定[J]. 天津农学院学报, 2009, 16(2): 9-11 |
| 作者姓名： | 黄俊轩 倪志明 桂毓 解瑞彬 刘艳军 杨静慧 吕成功 |
| 作者单位： | 1. 天津农学院园艺系,天津,300384 2. 天津市环境建设投资有限公司,天津,300381 3. 天津市武清区王庆坨镇政府农业办公室,天津,301713 |
| 基金项目： | 天津市科技支撑项目，天津农学院青年基金 |
| 摘 要： | 在以抗生素为筛选标记的基因转化中，确定适宜的筛选压力是提高基因转化率的前提。本研究以卡那霉素为筛选标记，在10、20、30、50和100mg／L的筛选压力下，根据金鱼草下胚轴外植体的生长表现、愈伤组织和芽的分化率确定适宜的筛选浓度。结果表明，适宜卡那霉素的筛选浓度为50mg／L。以此浓度为筛选压力进行遗传转化所获得的部分再生苗经Southernblot鉴定，均为转化株。 |
| 关 键 词： | 基因转化 金鱼草 卡那霉素 筛选压力 |
Confirmation of Selective Concentration of Kanamycin in Genetic Transformation for Antirrhinura majus L. |
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| HUANG Jun-xuan,NI Zhi-ming,GUI Yu,XIE Rui-bin,LIU Yan-jun,YANG Jing-hui,LV Cheng-gong. Confirmation of Selective Concentration of Kanamycin in Genetic Transformation for Antirrhinura majus L.[J]. Journal of Tianjin Agricultural University, 2009, 16(2): 9-11 | |
| Authors: | HUANG Jun-xuan NI Zhi-ming GUI Yu XIE Rui-bin LIU Yan-jun YANG Jing-hui LV Cheng-gong |
| Affiliation: | 1. Department of Horticulture, Tianjin Agricultural University, Tianjin 300384, China; 2. Tianjin Environment Development Inv. Co. Ltd., Tianjin 300191, China; 3. Agriculture office of Government of Wangqingtuo Town in Wuqing District of Tianjin, Tianjin 301713, China) |
| Abstract: | In order to confirm the optimum selective concentration of antibiotic in genetic transformation, kanamycin was selected as a case in this paper. It had been found that the optimum selective concentration of kanamycin was 50 mg/L according to the selective effects, such as the growth and the difference of explants of Antirrhinura majus L. on the medium with different concentrations of 10, 20, 30, 50 and 100 mg/L, respectively. Some shoots of transformation selected by 50 mg/L kanamycin were confirmed by Southern blot. |
| Keywords: | genetic transformation Antirrhinura majus L.： kanamycin selective concentration |
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