| 大白杜鹃组培与快繁研究——基本外植体和初代培养基筛选 |
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| 引用本文： | 刘燕,王济红,陈训,高贵龙.大白杜鹃组培与快繁研究——基本外植体和初代培养基筛选[J].种子,2010,29(8). |
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| 作者姓名： | 刘燕 王济红 陈训 高贵龙 |
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| 作者单位： | 1. 贵州省生物研究所,贵阳,550009
2. 贵州科学院,贵阳,550001 |
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| 基金项目： | 农业科技成果转化资金项目，贵州省重大科技攻关项目，贵州省体改转制项目 |
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| 摘 要： | 以大白杜鹃茎尖、茎段、种子为试验材料,对大白杜鹃进行了初代培养研究。结果表明:茎段比茎尖更适合作为芽诱导培养材料;基本培养基以1/4 MS为宜,适合芽诱导培养基配方为1/4 MS+ZT 0.5 mg/L+NAA 0.01 mg/L,芽诱导率在92%以上。种子萌发培养基配方也为1/4 MS+ZT 0.5 mg/L+NAA 0.01 mg/L,萌发率达100%。
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| 关 键 词： | 大白杜鹃 组培快繁 初代培养 |
Study on Tissue Culture and Rapid Propagation of Rhododendron decorum Screening the Basic Explants and Inducement Medium in Primary Culture |
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| LIU Yan,WANG Ji-hong,CHEN Xun,GAO Gui-long.Study on Tissue Culture and Rapid Propagation of Rhododendron decorum Screening the Basic Explants and Inducement Medium in Primary Culture[J].Seed,2010,29(8). |
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| Authors: | LIU Yan WANG Ji-hong CHEN Xun GAO Gui-long |
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| Institution: | LIU Yan1,WANG Ji-hong1,CHEN Xun2,GAO Gui-long2(1.Guizhou institute of Biology,Guiyang 550009,China,2.Guizhou Academy of Sciences,Guiyang 550001,China) |
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| Abstract: | This article mainly studied on primary culture of Rhododendron decorum with shoot tips,stems,seeds as testing materials.The results showed that: As explants,the stem segment was better than shoot tip,1 /4 MS was suitable for culturing explants.1 /4 MS + ZT 0.5 mg/L + NAA 0.01 mg/L was the basic culture medium for induction shoots,shoots induction rate was 92% or more.The suitable medium for seed germination was 1 /4 MS + ZT 0.5 mg/L + NAA 0.01 mg/L,and the germination rate could reach 100%. |
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| Keywords: | Rhododendron decorum tissue culture and rapid propagation primary culture |
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