| 土壤中棉花黄萎病菌快速检测技术研究 |
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| 引用本文： | 高峰,刘培源,马江峰,李晖,李国英.土壤中棉花黄萎病菌快速检测技术研究[J].植物病理学报,2009,39(3):310-313. |
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| 作者姓名： | 高峰 刘培源 马江峰 李晖 李国英 |
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| 作者单位： | 石河子大学新疆兵团绿洲生态农业重点实验室, 石河子 832003 |
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| 基金项目： | 国家优质棉基地建设项目 |
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| 摘 要： | Verticillium wilt,caused by Verticillium dahliae,is the most important disease of cotton.In this work,the previously developed defoliating(D) and nondefoliating(ND) V.dahliae-specific primers were adopted for detection of pathotypes of V.dahliae in soil by using nested PCR.The results showed that the detection assay was efficient when used in infested soil and was an useful technique for rapid and accurate assessment of soil contamination by V.dahliae.
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Studies on the detection of defoliating and nondefoliating pathotypes of Verticillium dahliae in Soils |
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| GAO Feng,LIU Pei-yuan,MA Jiang-feng,LI Hui,LI Guo-ying.Studies on the detection of defoliating and nondefoliating pathotypes of Verticillium dahliae in Soils[J].Acta Phytopathologica Sinica,2009,39(3):310-313. |
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| Authors: | GAO Feng LIU Pei-yuan MA Jiang-feng LI Hui LI Guo-ying |
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| Institution: | Key Laboratory of Oasis Ecology Agriculture, Shihezi University, Shihezi 832003, China |
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| Abstract: | Verticillium wilt,caused by Verticillium dahliae,is the most important disease of cotton.In this work,the previously developed defoliating(D) and nondefoliating(ND) V.dahliae-specific primers were adopted for detection of pathotypes of V.dahliae in soil by using nested PCR.The results showed that the detection assay was efficient when used in infested soil and was an useful technique for rapid and accurate assessment of soil contamination by V.dahliae. |
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| Keywords: | cotton Verticillium dahliae soil molecular detection |
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