| 番木瓜CYP79A2基因片段的克隆与序列分析 |
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| 引用本文： | 李泽友,沈文涛,言普,李亚丽,周鹏. 番木瓜CYP79A2基因片段的克隆与序列分析[J]. 热带生物学报, 2010, 1(1): 55-58 |
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| 作者姓名： | 李泽友 沈文涛 言普 李亚丽 周鹏 |
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| 作者单位： | 海南大学,农学院,海南,海口,570228;海南医学院,药学系,海南,海口,571101;中国热带农业科学院,热带生物技术研究所,海南,海口,571101;海南大学,农学院,海南,海口,570228;海南大学,农学院,海南,海口,570228;中国热带农业科学院,热带生物技术研究所,海南,海口,571101 |
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| 基金项目： | 中央级事业单位基本科研业务费 |
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| 摘 要： | 苯丙氨酸氮羟化酶是植物中催化异硫氰酸苄酯(BITC)生物合成的第1个酶。笔者以番木瓜嫩叶总RNA反转录得到的cDNA为模板,依据拟南芥(NM-120608)和油菜(EU877074)的CYP79A2基因序列,并结合番木瓜的全基因组序列草图设计引物,进行PCR扩增,得到了大小为923bp的基因片段,命名为CP-CYP79A2。生物信息学分析结果表明,其为番木瓜的CYP79A2基因片段。为进一步研究番木瓜BITC的生物合成调控打下了良好的基础。
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| 关 键 词： | 番木瓜 异硫氰酸苄酯 苯丙氨酸氮羟化酶 基因克隆 序列分析 |
Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya |
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| LI Ze-you,SHEN Wen-tao,YAN Pu,LI Ya-li,ZHOU Peng. Cloning and Sequence Analysis of CYP79A2 Gene Fragment in Papaya[J]. Journal of Tropical Biology, 2010, 1(1): 55-58 |
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| Authors: | LI Ze-you SHEN Wen-tao YAN Pu LI Ya-li ZHOU Peng |
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| Affiliation: | 1. College of Agriculture, Hainan University, Haikou 570228, China; 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 3 Hainan Medical College, Haikou 571101, China) |
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| Abstract: | L-phenylalanine N-hydroxylase is the first enzyme which catalyze the biosynthesis of benzylisothiocyanate （BITC） in plant. According to the sequences of CYP79A2 gene of Arabidopsis thaliana （NM120608） and Brassica rapa（EU877074）from GenBank and combining with the genome draft of the transgenic tropical fruit tree papaya, specific primers were designed and RT-PCR was used to amplify partial sequences of CYP79A2 gene from papaya young leaves. The sequence includes 923bp, which named CPCYP79A2. Bioinformatics analysis results showed that the frgment is the partial sequences of CYP79A2 gene of papaya. The data set a strong basis for the study of the biosynthetic regulation of BITC in papaya. |
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| Keywords: | papaya benzylisothiocyanate （ BITC ） L-phenylalanine N-hydroxylase cloning sequence analysis |
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