水栒子组织培养快繁体系研究

以水栒子（Cotoneaster multiflorus Bunge.）的新梢带侧芽茎段为外植体,研究了不同激素组合对其外植体诱导、丛生芽分化增殖以及试管苗生根的影响。结果表明：芽诱导最适培养基为MS＋BA0.8＋NAA0.04,诱导率为68%;分化增殖最佳培养基是MS＋6-BA1.2＋NAA0.04,增殖倍数为4.53;最佳生根培养基是1/2MS＋IBA0.4＋NAA0.04,生根率为91%;试管苗移栽的最佳基质组合及配比为沙子∶熟表土∶草炭土∶果渣=3∶3∶2∶2,成活率达91%。

Tissue Culture and Rapid Propagation System of Cotoneaster multi f lorus

Lateral buds with stem segments for new shoots of Cotoneaster multiflorus were taken as explants ;the effects of different combinations of hormones on its explant induction ,the differentiation proliferation of multiple buds ,rooting of plantlet were studied .Result shows that ：the optimum medium for bud induction is MS ＋ BA0 .8＋ NAA0 .04 ,its induction rate being 68% ;the optimal medium for differentiation proliferation is MS ＋ 6 -BA1 .2 ＋ NAA0 .04 ,the proliferation multiples being 4 .53 ;the optimal rooting medium is 1/2MS ＋ IBA0 .4 ＋NAA0 .04 ,the rooting rate being 91% ;the optimal combination ratio of the transplanted plantlets is ：sand∶ma-ture topsoil∶turfy soil∶fruit marc = 3∶3∶2∶2 ,the survival rate being 91% .