| 培养条件对酸枣叶片不定梢再生率的影响 |
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| 引用本文： | 孙清荣,刘庆忠,孙洪雁.培养条件对酸枣叶片不定梢再生率的影响[J].果树学报,2002,19(1):24-26. |
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| 作者姓名： | 孙清荣 刘庆忠 孙洪雁 |
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| 作者单位： | 山东省果树生物技术育种重点实验室,山东省果树研究所,山东,泰安,271000 |
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| 摘 要： | 以酸枣无菌苗叶片为外植体,选用基本培养基ＮＮ６９和ＷＰＭ,外源激素包括ＴＤＺ、ＩＡＡ、ＩＢＡ和ＮＡＡ,暗培养时间为２、３、４周。采用正交实验设计方法,研究了基本培养基、外源激素及暗培养时间对叶片不定梢再生的影响。结果表明在附加ＴＤＺ１~３ｍｇ/Ｌ的ＷＰＭ和ＮＮ６９上都可诱导不定梢再生,但ＷＰＭ比ＮＮ６９更有效。暗培养时间对不定梢再生也有影响,暗培养３周比２、４周更有利于提高叶片的不定梢再生率。在附加ＴＤＺ１ｍｇ/Ｌ、ＩＡＡ０．１ｍｇ/Ｌ的ＷＰＭ培养基上,暗培养３周后转到光下培养,获得的不定梢再生率达８７．５%。
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| 关 键 词： | 酸枣 无菌苗叶片 不定梢再生 |
| 文章编号： | 1009-9980-(2002)-01-024-03 |
| 修稿时间： | 2001年2月28日 |
Effects of Culture Condititions on Shoot Regeneration from Leaves of Sour Jujube (Zizyphus spinosus Hu.) |
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| Sun Qingrong,Liu Qingzhong,and Sun Hongyan.Effects of Culture Condititions on Shoot Regeneration from Leaves of Sour Jujube (Zizyphus spinosus Hu.)[J].Journal of Fruit Science,2002,19(1):24-26. |
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| Authors: | Sun Qingrong Liu Qingzhong and Sun Hongyan |
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| Abstract: | Leaves of sour jujube(Zizyphus spinosus Hu.) in vitro were selected as explants.Experiment factors included basicmedia NN69 and WPM, hormone TDZ,IAA,IBA and NAA.Experiment design was orthogonal test.Effects of basic medium,hormone and dark time on shoot regeneration from leaves of sour jujube were studied.Results showed that shoot regenerationwas obtained on both WPM and NN69 media, but WPM was more effective than NN69. Dark culture time also influencedshoot regeneration,3 weeks under dark was more effective in inducing shoot regeneration than 2 or 4 weeks.The highest re-generation rate was obtained on the medium WPM supplemented with 1 mg/L TDZ, 0.1mg/L IAA, under dark 3 weeks laterin the light,regeneration rate reached 87.5%. |
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| Keywords: | Sour jujube Leaves in vitro Shoot regeneration |
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