燕麦属牧草根尖组织染色体观察方法的优化研究

针对通常采用的燕麦属牧草染色体观察的去壁低渗法，对该方法中的预处理和解离等环节进行了优化，即预处理用对二氯苯在20℃下处理4h，固定，酸解离用1mol／LHCl在60℃下处理10min，酶解离用20g／L纤维素酶溶液在25℃下处理20min，最后染色压片。通过研究发现优化后试验步骤简化，成本降低，且观察效果良好。

Study on optimization of observing method for chromosome of root-tip tissue in Avena

This study was conducted to optimize both the methods of pretreatment and digestion of the cell wall by enzyme in a wall degradation hypotonic treatment,which was usually accepted to survey chromosome number in oat(Avena)root tip tissue.Basing on the optimized method,the root tip tissues were treated with p-dichlorobenzene for 4 hours at 20 ℃,then fixed the tissues.After being digested by 1 mol/L HCl for 10 minutes at 60 ℃,they were digested by the 20 g/L cellulolyic enzyme for 20 minutes at 25 ℃.The last step was dyeing and pressing the slide.Since the hypoosmotic step in usual wall degradation hypotonic treatment method was omitted,and the two enzyme digestion steps including pectotytic and cellulolyic enzyme were combined into a cellulolyic enzyme digestion,the method was simplified and its cost reduced.By so doing,an accepted method of chromosome observation for oat root tip tissue was obtained.